{"product_id":"biolegend-405243","title":"Biolegend, 405243, APC Streptavidin (High Concentration), 500μg","description":"\u003cp\u003eStreptavidin binds to biotin with high affinity. Streptavidin-APC is useful for detecting biotinylated antibodies. The excitation of APC by 600-635 nm laser light induces a fluorescence emission maximum of 660 nm.\u003cbr\u003e\n500μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse, Rat, All Species\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: Streptavidin is conjugated with APC under optimal conditions.\u003cbr\u003e\nConcentration: 1.0 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The Streptavidin-APC solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested ICFC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this Streptavidin-APC is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the APC conjugation. For immunofluorescent staining, the recommended use of this reagent is ≤ 0.06 µg per million cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: Streptavidin-Allophycocyanin (APC) is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-APC is 320 kD and Streptavidin alone is 52 kD.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Kamimura D, et al. 2006. J. Immunol. 177:306. Andoniou CE, et al. 2005. Nature Immunology 6:1011. Chou WC,et al.2006.Blood108:3005. PubMed Malagolini N, et al. 2009. Glycobiology. 19:172. PubMed Kroeger KM, et al. 2009. J. Leukocyte Biol. PubMed Imada M, et al. 2009. Int. Immunol. 21:1151. PubMed Yi Y, et al. 2010 J. Biol Chem. 285:21233. PubMed King IL, et al. 2010. J. Immunol. 185:6138. PubMed Yokoyama T, et al. 2011. Int Immunol. PubMed Rusert P, et al. 2011. J. Exp Med. 208:1419. PubMed Bollig N, et al. 2012. PNAS. 109:8664. PubMed Nguyen V, et al. 2012. J. Immunol. 189:1081. PubMed Jager NA, et al. 2012. J Nucl Med. 53:1229. PubMed Liberman AC, et al. 2012. Mol Immunol. 50:220. PubMed Ogawa H, et al. 2012. Biochem Biophys Res Commun. 420:114. PubMed Ludin A, et al.2012. Nat Immunol. 13:1072. PubMed Sugiura D, et al. 2012. PLoS One. 7:e44770. PubMed Tani-Ichi S, et al. 2012. PNAS. 110:612. PubMed Jin L, et al. 2013. PNAS. 110:3907. PubMed Ota T, et al. 2013. J. Immunol. 191:3179. PubMed Fu X, et al. 2013. PNAS. 110:17982. PubMed Ryan SO, et al. 2014. Glycobiology. 24:362. PubMed Lyngaa R, et al. 2014. Clin Cancer Res. 20:1768. PubMed Pilling D, et al. 2014. PLoS One. 9:93730. PubMed Matthews JA, et al. 2014. PLoS One. 9:97707. PubMed Owen KA, et al. 2014. PLoS Pathog. 10:1004159. PubMed Duek A, et al. 2014. Blood. 123:3943. PubMed Madireddi S, et al. 2014. J Exp Med. 211:1433. PubMed\u003cbr\u003e\nProduct Citations: Yu H, et al. 2023. Nat Commun. 14:1058. PubMed Kristensen NP, et al. 2022. J Clin Invest. 132:. PubMed Kim DJ, et al. 2020. J Virol. :94. PubMed Han Y, et al. 2019. J Clin Invest. 130:26. PubMed Brunk F, et al. 2021. Eur J Immunol. 51:2651. PubMed\u003cbr\u003e\nGene ID: NA\u003cbr\u003e\nUniProt: View information about Biotin on UniProt.org\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Streptavidin-Allophycocyanin, SAv-APC\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866503696553,"sku":"405243","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-405243","provider":"Iright","version":"1.0","type":"link"}