{"product_id":"biolegend-405416","title":"Biolegend, 405416, Alexa Fluor® 647 Goat anti-rat IgG (minimal x-reactivity) Antibody, 100μg","description":"\u003cp\u003eGoat anti-rat IgG antibody reacts primarily with the heavy chains of rat IgG, but also partially binds to the light chains common to most rat immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rabbit, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other immunoglobulins from other species.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Rat\u003cbr\u003e\nAntibody Type: Polyclonal\u003cbr\u003e\nHost Species: Goat\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested IHC-F - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 2.5 - 5.0 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm \/ 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: This goat anti-rat IgG antibody is useful for direct immunofluorescent staining or as a second step reagent for indirect immunofluorescent staining when used in conjunction with primary antibodies.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Zornetzer GA, et al. 2010. J. Virol. 84:11297. PubMed Frank NY, et al. 2011. Cancer Res. 71:1474. (IHC) PubMed Baba T, et al. 2020. eLife. 9:e54257. (IHC-P)\u003cbr\u003e\nProduct Citations: Yu L, et al. 2022. Cancer Sci. 113:3980. PubMed Zhang Z, et al. 2022. Cancer Gene Ther. 29:722. PubMed Chandler A, et al. 2022. J Orthop Res. 40:1918. PubMed Ghanei Z, et al. 2020. Journal of Cellular Physiology. 235:7840. PubMed Kim H, et al. 2020. ACS Nano. 14:6519. PubMed Duan H, et al. 2020. JCI Insight. 5:00. PubMed Zhang P, et al. 2020. Nat Commun. 11:2487. PubMed Cong L, et al. 2021. Breast Cancer Res. 23:51. PubMed Hu J, et al. 2017. Cell Death Dis. 10.1038\/cddis.2017.72. PubMed\u003cbr\u003e\nRRID: AB_2562967 (BioLegend Cat. No. 405416)\u003cbr\u003e\nGene ID: 295279\u003cbr\u003e\nUniProt: View information about IgG on UniProt.org\u003cbr\u003e\nClone: Poly4054\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nIsotype: Goat Polyclonal IgG\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866468143273,"sku":"405416","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-405416","provider":"Iright","version":"1.0","type":"link"}