{"product_id":"biolegend-405708","title":"Biolegend, 405708, Alexa Fluor® 647 anti-mouse IgD Antibody, 100μg","description":"\u003cp\u003eSurface IgD is an important B cell differentiation marker.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested 3D IHC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg\/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm \/ 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: The 11-26c.2a antibody reacts with immunoglobulin D in all tested mouse haplotypes. The antibody binds membrane IgD expressed on most B cells. The 11-26c.2a antibody neither induces proliferation of splenic B cells nor induces B cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections2,3, and spatial biology (IBEX)10,11.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Nitschke L, et al. 1993. P. Natl. Acad. Sci. USA 90:1887. (FC) Weih D, et al. 2001. J. Immunol. 167:1909. (IHC) Koni PA, et al. 2001. J. Exp. Med. 193:741. (IHC) Ahuja A, et al. 2007. J. Immunol. 179:3351. (FC) PubMed Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC) Good-Jacobson KL, et al. 2010. Nat. Immunol. 11:535. (FC) PubMed Tomayko MM, et al. 2010. J. Immunol. 185:7146. PubMed Park SY, et al. 2013. J. Immunol. 190:1094. PubMed Rouaud P, et al. 2014. J Exp Med. 211:975. PubMed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed\u003cbr\u003e\nProduct Citations: Soon MSF, et al. 2020. Nat Immunol. 1.984027778. PubMed Yazicioglu YF, et al. 2023. Nat Immunol. 24:991. PubMed Chan T, et al. 2009. J Immunol. 183:3139. PubMed Vanderleyden I, et al. 2020. Cell Rep. 30:611. PubMed Wang L, et al. 2019. Cell Rep. 29:1848. PubMed Gallman AE, et al. 2021. Sci Immunol. 6:. PubMed Lindner J, et al. 2013. Mol Cell Biol. 33:4628. PubMed Rodda LB et al. 2018. Immunity. 48(5):1014-1028 . PubMed Shi B, et al. 2018. J Immunol. 200:586. PubMed Gatto D, et al. 2011. J Immunol. 187:4621. PubMed Vu T, et al. 2011. J Immunol. 187:5032. PubMed Biram A, et al. 2020. Cell Rep. 30:1910. PubMed Adori M, et al. 2018. J Immunol. 200:775. PubMed Lee JL, et al. 2022. Aging Cell. 21:e13692. PubMed Avery DT, et al. 2018. J Exp Med. 215:2073. PubMed He Y, et al. 2022. J Exp Med. 219:. PubMed Duan L, et al. 2021. Immunity. 54:2256. PubMed Xu H, et al. 2020. Immunity. 51(4):696-708.e9.. PubMed Siamishi I, et al. 2020. Cell Reports. 31(11):107756. PubMed Ogbe A, et al. 2015. J Biol Chem. 290: 20455-20465. PubMed Crouse B, et al. 2020. NPJ Vaccines. 0.277083333. PubMed Suan D et al. 2017. Immunity. 47(6):1142-1153 . PubMed Zhong MC, et al. 2021. J Exp Med. 218:. PubMed Volkmann C, et al. 2016. EMBO J. 35: 2371 - 2381. PubMed Kishi Y, et al. 2012. Proc Natl Acad Sci U S A. 109:7811. PubMed Vacchio MS, et al. 2020. Immunity. 51(3):465-478. PubMed Stephens WZ, et al. 2021. Cell Rep. 37:109916. PubMed Dotsey E, et al. 2017. Sci Rep. 7:42584. PubMed Fra-Bido S, et al. 2021. STAR Protocols. 2(3):100499. PubMed Fleig S, et al. 2022. Nat Commun. 13:2022. PubMed Cheng Q, et al. 2018. PLoS Pathog. 14:e1007440. PubMed Silva-Cayetano A, et al. 2020. Med. 2(3):243-262.e8. PubMed Lederer K, et al. 2020. Immunity. 53(6):1281-1295.e5. PubMed Lahoud M, et al. 2011. J Immunol. 187:842. PubMed Monzon-Casanova E, et al. 2021. Eur J Immunol. 51:2266. PubMed Jandl C, et al. 2017. Nat Commun. 10.1038\/ncomms14647. PubMed Maity P, et al. 2015. Sci Signal. 8: ra93. PubMed Dahlgren M, et al. 2015. J Immunol. 194:5187. PubMed Barwick BG, et al. 2018. Nat Commun. 9:1900. PubMed Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed\u003cbr\u003e\nRRID: AB_893529 (BioLegend Cat. No. 405707) AB_893528 (BioLegend Cat. No. 405708)\u003cbr\u003e\nStructure: Ig family\u003cbr\u003e\nDistribution: B cells\u003cbr\u003e\nFunction: B cell differentiation\u003cbr\u003e\nCell Type: B cells\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nGene ID: 380797\u003cbr\u003e\nUniProt: View information about IgD on UniProt.org\u003cbr\u003e\nClone: 11-26c.2a\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Immunoglobulin D\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860784238761,"sku":"405708","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-405708","provider":"Iright","version":"1.0","type":"link"}