{"product_id":"biolegend-407115","title":"Biolegend, 407115, Alexa Fluor® 647 anti-mouse IgG2a Antibody, 25μg","description":"\u003cp\u003eThe RMG2a-62 monoclonal antibody reacts with immunoglobulin G2a and G2c (IgG2a, IgG2c) and in all tested mouse haplotype (Igh-a and b). It does not react with other isotypes. The RMG2a-62 monoclonal antibody may be used as primary or secondary reagent for ELISA or immunofluorescent analysis.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Mouse Ig cocktail\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested IHC-P - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1 - 10 µg\/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm \/ 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Gavin AL, et al.2006. Science 314:1936. Podolin PL, et al. 2008. J. Immunol. 180:7989. (IP) PubMed\u003cbr\u003e\nProduct Citations: Kodali S, et al. 2022. J Immunol. 208:1085. PubMed Pan R, et al. 2022. Cell. 185:1521. PubMed\u003cbr\u003e\nRRID: AB_2563482 (BioLegend Cat. No. 407115) AB_2563483 (BioLegend Cat. No. 407116)\u003cbr\u003e\nGene ID: 668478\u003cbr\u003e\nUniProt: View information about IgG2a on UniProt.org\u003cbr\u003e\nClone: RMG2a-62\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Immunoglobulin G2a\u003cbr\u003e\nIsotype: Rat IgG\u003cbr\u003e\nQ: IgG2a gene is deleted in some mouse strains, which ones are they?\u003cbr\u003e\nA: IgG2a gene is deleted in C57Bl\/6, C57Bl\/10, SJL, and NOD mice. It is replaced with IgG2c gene instead.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866198626473,"sku":"407115","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-407115","provider":"Iright","version":"1.0","type":"link"}