{"product_id":"biolegend-408103","title":"Biolegend, 408103, Biotin anti-phycoerythrin (PE) Antibody, 25μg","description":"\u003cp\u003eThe PE001 antibody reacts with both phycoerythrin (PE) alone and PE conjugated with antibody or streptavidin, as well as PE tandem dyes such as PE\/Cyanine5, PE\/Cyanine5.5 and PE\/Cyanine7. When PE001 antibody is used in a three-step staining procedure or separation of PE-antibody labeled cells, it does not quench PE fluorescence or react with tested mouse and human cells. The PE001 antibody can be used for separation of PE-antibody labeled cells or as a secondary step to amplify PE conjugated primary antibody signals (i.e. biotinylated PE001 as secondary step, followed by Streptavidin-PE).\u003cbr\u003e\n25μg\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Phycoerythrin\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Use at concentrations ≤ 0.5 µg\/test. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Taylor RT, et al. 2007. J. Immunol. 178:5659.\u003cbr\u003e\nProduct Citations: Cacialli P, et al. 2022. EMBO J. 41:e108536. PubMed\u003cbr\u003e\nRRID: AB_2237499 (BioLegend Cat. No. 408103) AB_2168923 (BioLegend Cat. No. 408104)\u003cbr\u003e\nGene ID: NA\u003cbr\u003e\nUniProt: View information about Phycoerythrin on UniProt.org\u003cbr\u003e\nClone: PE001\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: PE\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: How many biotin molecules are per antibody structure?\u003cbr\u003e\nA: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866161107113,"sku":"408103","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-408103","provider":"Iright","version":"1.0","type":"link"}