{"product_id":"biolegend-408303","title":"Biolegend, 408303, Biotin anti-FITC Antibody, 25μg","description":"\u003cp\u003eThe FIT-22 antibody reacts with both fluorescein isothiocyanate (FITC) alone and FITC conjugated with antibody or streptavidin. When FIT-22 antibody is used in a three-step staining procedure or separation of FITC-antibody labeled cells, it does not quench FITC fluorescence or react with tested mouse and human cells. The FIT-22 antibody can be used for separation of FITC-antibody labeled cells or as a secondary step to amplify FITC conjugated primary antibody signals (i.e. biotinylated FIT-22 as secondary step, followed by Streptavidin-FITC).\u003cbr\u003e\n25μg\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: FITC conjugated mouse IgG.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nProduct Citations: Bhadra R, et al. 2013. Infect Immun . 81:3414. PubMed Zhao L, et al. 2020. Pharm Biol. 1085:58. PubMed Hwang S, et al. 2016. J Exp Med. 213: 1799 - 1818. PubMed\u003cbr\u003e\nRRID: AB_528899 (BioLegend Cat. No. 408304)\u003cbr\u003e\nGene ID: NA\u003cbr\u003e\nUniProt: View information about FITC on UniProt.org\u003cbr\u003e\nClone: FIT-22\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Fluorescein isothiocyanate\u003cbr\u003e\nIsotype: Mouse IgG1, λ\u003cbr\u003e\nQ: How many biotin molecules are per antibody structure?\u003cbr\u003e\nA: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866089574569,"sku":"408303","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-408303","provider":"Iright","version":"1.0","type":"link"}