{"product_id":"biolegend-409004","title":"Biolegend, 409004, PE anti-Biotin Antibody, 100μg","description":"\u003cp\u003eBiotin (vitamin B7 or vitamin H) is essential for cell growth and the metabolization of fat and protein. It is also often used to tag a molecule of interest. The anti-biotin antibody can be used as a second-step reagent for detecting biotinylated targets.\u003cbr\u003e\n100μg\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Biotinylated limulus hemocyanin\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: FC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for relevant formats) include: immunoprecipitation and ELISA.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Uccellini MB, et al. 2008. J. Immunol. 181:5875. Green NM, et al. 2012. J Biol Chem. 287:39789. PubMed.\u003cbr\u003e\nProduct Citations: Hossain MA, et al. 2022. Cell Rep. 39:111021. PubMed Guo H, et al. 2022. Int J Oncol. 61: . PubMed Paprckova D, et al. 2022. Front Immunol. 13:1009198. PubMed Tatovic D, et al. 2015. J Immunol. 195: 386 - 392. PubMed Howe EN, et al. 2020. Nat Commun. 2.553472222. PubMed Joly P, et al. 2017. PLoS One. 12(6):e0180568. PubMed Chan W, et al. 2014. Leukemia. . PubMed Nakatsugawa M, et al. 2016. Sci Rep. 6:23821. PubMed Zou F, et al. 2019. Nat Commun. 10:4109. PubMed\u003cbr\u003e\nRRID: AB_10645482 (BioLegend Cat. No. 409003) AB_10641847 (BioLegend Cat. No. 409004)\u003cbr\u003e\nLigand\/Receptor: Avidin, Streptavidin\u003cbr\u003e\nGene ID: NA\u003cbr\u003e\nUniProt: View information about Biotin on UniProt.org\u003cbr\u003e\nClone: 1D4-C5\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Vitamin B7, Vitamin H\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860852756649,"sku":"409004","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-409004","provider":"Iright","version":"1.0","type":"link"}