{"product_id":"biolegend-421205","title":"Biolegend, 421205, ELISA Assay Diluent B (5X), 100ml","description":"\u003cp\u003eELISA Assay Diluent B is a 5x concentrated phosphate buffered saline solution containing (pre-screened) bovine serum albumin. This buffer is specifically formulated and tested for ELISA applications, such as blocking antibody-coated ELISA plate microwells, diluting ELISA standards and samples, and diluting biotinylated detection antibodies or tertiary reagents (especially Avidin-HRP or Streptavidin-HRP). The diluent contains a preservative compatible with peroxidase mediated enzyme activity.\u003cbr\u003e\n100ml\u003cbr\u003e\nStorage \u0026amp; Handling: This solution can be stored unopened between 2°C and 8°C. After opening, it is recommended to use within 30 days or to be aliquoted and stored at -20°C (frost-free) or -70°C for long term storage. Warm ELISA reagents to room temperature (18-25°C) before use.\u003cbr\u003e\nApplication: ELISA\u003cbr\u003e\nRecommended Usage: Prior to use, dilute the ELISA Assay Diluent B (5X) in PBS, pH 7.4. PBS recipe: 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, and 0.2 g KCl. Add deionized water to 1.0 L, filter through 0.2 µm filter, pH to 7.4\u003cbr\u003e\nProduct Citations: Lim J, et al. 2020. Sci Rep. 10:14897. PubMed\u003cbr\u003e\nGene ID: NA\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: ELISA assay diluent, ELISA diluent\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864111009961,"sku":"421205","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-421205","provider":"Iright","version":"1.0","type":"link"}