{"product_id":"biolegend-427508","title":"Biolegend, 427508, Puromycin dihydrochloride, 50mg","description":"\u003cp\u003ePuromycin dihydrochloride is a naturally occurring amino nucleoside antibiotic produced by Streptomyces alboniger . Comprised of a nucleoside covalently bound to an amino acid, Puromycin mimics the 3′ end of aminoacylated tRNAs and thus inhibits protein synthesis by ribosome-catalyzed incorporation into elongating nascent chains, which results in premature termination of translation. It is used as a selection agent in mammalian cell cultures for cell lines that express a puromycin resistance via puromycin N-acetyl-transferase (pac).  Puromycin immunodetection with antibodies such as BioLegend’s clone 2A4 allows the direct evaluation of translational activity in single cells by immunofluorescence microscopy and flow cytometry.\u003cbr\u003e\n50mg\u003cbr\u003e\nFormulation: Lyophilized\u003cbr\u003e\nPreparation: Solubility: 50-100 mg\/mL in distilled deionized water, PBS or DMSO.\u003cbr\u003e\nStorage \u0026amp; Handling: Store lyophilized powder at -20°C. When reconstituted, aliquot and store at -20°C. Use reconstituted material within 6 months to avoid loss of potency.\u003cbr\u003e\nApplication: Cell Culture - Verified\u003cbr\u003e\nRecommended Usage: Working concentrations of Puromycin dihydrochloride for cell-based assays range from 0.2 – 10 μg\/mL. It is recommended that the reagent be titrated for each experiment to determine optimal performance.\u003cbr\u003e\nStructure: C22 H29N7O5 · 2HCl. MW = 544.43 g\/mol\u003cbr\u003e\nFunction: Protein synthesis inhibitor, antibiotic\u003cbr\u003e\nAntigen References: Arguello R, et al. 2020. Cell Metabolism. 32:1063-1075. Nathans D, et al. 1964. PNAS.51:585-92. Miyamoto-Sato E, et al. 2000. Nucleic Acids Res. 28:1176-82. Schmidt EK, et al. 2009. Nat Methods. 6:275-7.\u003cbr\u003e\nGene ID: NA\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Protein Synthesis Inhibitor, Puromycin 2HCl, C22H31Cl2N7O5\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866481316009,"sku":"427508","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-427508","provider":"Iright","version":"1.0","type":"link"}