{"product_id":"biolegend-430226","title":"Biolegend, 430226, FluoroSpot MAX™ Deluxe Set Human TNF-α, 5plates","description":"\u003cp\u003eHuman TNF-α (Tumor Necrosis Factor α), also known as Cachectin, Cytotoxic Factor (CF), is a cytokine which has important roles in regulating a wide range of biological functions from immune activation, cellular differentiation, proliferation, apoptosis, and energy metabolism. TNF-α is expressed and secreted by macrophages, neutrophils, T-cells, NK cells, and various other cell types. TNF-α can bind to two receptors: TNFR1 and TNFR2. Signaling through TNFR1 is mostly pro-inflammatory, while TNFR2 signaling is mainly anti-inflammatory and proliferative. Because of its diverse functions, dysregulation or TNF-α expression and activity has been implicated in many diseases from autoimmune, cancer, to neurodegeneration.  BioLegend’s FluoroSpot MAX™ Deluxe Set contains pre-optimized essential reagents, buffers, and solutions for the detection of human TNF- α secreting cells. In this assay, anti-human TNF- α capture antibodies are coated on a clear-bottom microplate. Cells (stimulated or unstimulated) are added to the wells and incubated, allowing secreted TNF- α to bind to nearby capture antibodies. After incubation, cells are removed, and biotinylated detection antibodies are added followed by Streptavidin-PE. The resulting red spots represent the “footprint” of an individual TNF- α-secreting cell, allowing for precise quantification of cytokine-producing cells in the sample.\u003cbr\u003e\n5plates\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nApplication: FluoroSpot\u003cbr\u003e\nCell Type: B cells, Eosinophils, Macrophages, Mast cells, Neutrophils, T cells\u003cbr\u003e\nBiology Area: Cancer Biomarkers, Immuno-Oncology, Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines, Innate Immune Signaling\u003cbr\u003e\nGene ID: 7124\u003cbr\u003e\nUniProt: View information about TNF-alpha on UniProt.org\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Tumor Necrosis Factor α (TNF-α), Cachectin, Cytotoxic Factor (CF), Hemorrhagic Factor, Macrophage Cytotoxic Factor (MCF)\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864320233641,"sku":"430226","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-430226","provider":"Iright","version":"1.0","type":"link"}