{"product_id":"biolegend-502001","title":"Biolegend, 502001, Biotin anti-human IL-13 Antibody, 50μg","description":"\u003cp\u003eIL-13 is an immunoregulatory cytokine produced primarily by activated Th2 lymphocytes. IL-13 shares 30% amino acid sequence homology with IL-4 and demonstrates similar biological activities. The biological activities of IL-13 include: suppression of macrophage cytotoxic activity, upregulation of IL-1RA expression, and suppression of proinflammatory cytokine secretion. The poly5020 polyclonal antibody reacts with human interleukin-13 (IL-13).\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Polyclonal\u003cbr\u003e\nHost Species: Rabbit\u003cbr\u003e\nImmunogen: Recombinant human IL-13\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.2% BSA and 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.\u003cbr\u003e\nApplication: ELISA Detection, ELISPOT Detection\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by ELISA assay. As a detection antibody in ELISA assays, dilute 60 µl of this biotinylated antibody into 12 ml of detection buffer for one 96 well microtiter plate assay. To obtain a linear standard curve, serial dilutions of human IL-13 recombinant protein ranging from 2000 to 8 pg\/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: ELISA Detection or ELISPOT Detection: The biotinylated Poly5020 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified JES10-5A2 (Cat. No. 501902\/501910) antibody as the capture antibody. This product may contain other non-IgG subtypes.\u003cbr\u003e\nAdditional Product Notes: This antibody can be used for 4 plates.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Bailer, R.T., et al.., 1999. J. Immunol.. 162 :7534. Arva, E., et al.., 1999. Scand. J. Immunol.. 49:411.\u003cbr\u003e\nProduct Citations: Yan J, et al. 2020. Cell Rep. 107820:31. PubMed\u003cbr\u003e\nRRID: AB_2124285 (BioLegend Cat. No. 502001)\u003cbr\u003e\nStructure: Cytokine; 12.5 kD\u003cbr\u003e\nFunction: Suppression of macrophage cytotoxic activity; upregulation of IL-1RA expression; suppression of IL-1, IL-6, IL-8, IL-10, IL-12, chemokines MCP, MIP-1; differentiation of B cells, monocytes\u003cbr\u003e\nBioactivity: Suppression of macrophage cytotoxic activity; upregulation of IL-1RA expression; suppression of IL-1, IL-6, IL-8, IL-10, IL-12, chemokines MCP, MIP-1; differentiation of B cells, monocytes\u003cbr\u003e\nCell Sources: Activated T helper cells, CD8+ T cells, mast cells, NK cells\u003cbr\u003e\nCell Targets: B cells, monocytes, macrophages, basophils\u003cbr\u003e\nReceptors: Moderate affinity IL-13Rα1; high affinity heterodimer IL-13Rα1\/IL-4Rα also binds IL-4; both receptor complexes have γ-subunit in common with IL-2R, IL-4R, IL-7R, IL-15R ; high affinity IL-13Rα2 incapable of signaling\u003cbr\u003e\nCell Type: Tregs\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines\u003cbr\u003e\nAntigen References: 1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. Hilton, D., et al. 1996. P. Natl. Acad. Sci. USA 93:497. 3. Obiri, N., et al. 1995. J. Biol. Chem. 270:8797. 4. Ochensberger, B., et al. 1996. Blood 88:3028.\u003cbr\u003e\nRegulation: IL-13Rα2 possible antagonist\u003cbr\u003e\nGene ID: 3596\u003cbr\u003e\nUniProt: View information about IL-13 on UniProt.org\u003cbr\u003e\nClone: Poly5020\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Interleukin-13, NC30\u003cbr\u003e\nIsotype: Rabbit Polyclonal IgG\u003cbr\u003e\nQ: How many biotin molecules are per antibody structure?\u003cbr\u003e\nA: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866470666409,"sku":"502001","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-502001","provider":"Iright","version":"1.0","type":"link"}