{"product_id":"biolegend-502102","title":"Biolegend, 502102, Purified anti-human G-CSF Antibody, 500μg","description":"\u003cp\u003eGranulocyte - colony stimulating factor (G-CSF) is a potent stimulator of bone marrow cells especially those of neutrophilic granulocyte lineage. In addition, G-CSF can enhance the survival and activate the immunological functions of mature neutrophils. G-CSF is produced primarily by monocytes and macrophages upon activation by endotoxin, TNF-alpha or IFN-gamma.\u003cbr\u003e\n500μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: E. coli-expressed, recombinant human G-CSF\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: ELISA Capture - Quality testedIHC - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 1-4 µg\/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: ELISA or ELISPOT Capture1,2: The purified BVD13-3A5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated BVD11-37G10 antibody (Cat. No. 506702) as the detecting antibody. The LEAF™ purified antibody is suggested for ELISPOT capture.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Abrams J. 1995. Curr. Prot. Immunol.. John Wiley and Sons New York. Unit 6.20. Abrams J, et al. 1992. Immunol. Rev. 127:5. Andersson J, et al. 1994. Immunology 83:16. Sander B, et al. 1991. Immunol. Rev. 119:65. Andersson U, et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.\u003cbr\u003e\nProduct Citations: Hollmén M, et al. 2016. Oncoimmunology. e1115177:5. PubMed\u003cbr\u003e\nRRID: AB_315207 (BioLegend Cat. No. 502101) AB_2083733 (BioLegend Cat. No. 502102)\u003cbr\u003e\nStructure: Cytokine; 21 kD (Mammalian)\u003cbr\u003e\nBioactivity: Growth\/differentiation\/activating factor for neutrophils; proliferation\/migration endothelial cells cells; synergistic with IL-3 growth of hematopoietic progenitors\u003cbr\u003e\nCell Sources: Macrophages, monocytes, neutrophils, fibroblasts, endothelial cells, bone marrow stromal cells\u003cbr\u003e\nCell Targets: Hematopoietic progenitors of neutrophil\/granulocyte lineage, endothelial cells\u003cbr\u003e\nReceptors: CD114 (G-CSFR)\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines\u003cbr\u003e\nAntigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. Demetri G, et al. 1991. Blood 78:2791. 3. Moore M. 1991. Annu. Rev. Immunol. 9:159. 4. Negrin R, et al. 1992. Adv. Pharmacol. 23:263.\u003cbr\u003e\nRegulation: Upregulated by IL-1, IL-17, TNF-α, IFN-γ, GM-CSF; downregulated by prostaglandin E2\u003cbr\u003e\nGene ID: 1441\u003cbr\u003e\nUniProt: View information about G-CSF on UniProt.org\u003cbr\u003e\nClone: BVD13-3A5\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Granulocyte-Colony Stimulating Factor, CSF-β, Pluripoietin, Granulocytic neutrophil colony stimulating activity (G-CSA), Macrophage-granulocyte inducer-2 (MGI-2)\u003cbr\u003e\nIsotype: Rat IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862929526953,"sku":"502102","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-502102","provider":"Iright","version":"1.0","type":"link"}