{"product_id":"biolegend-502304","title":"Biolegend, 502304, Biotin anti-human GM-CSF Antibody, 500μg","description":"\u003cp\u003eGranulocyte\/macrophage - colony stimulating factor (GM-CSF) is a hematopoietic factor that is produced by activated T cells, B cells, mast cells, macrophages, fibroblasts, and endothelial cells. In addition to supporting colony formation of granulocyte\/macrophage progenitors, GM-CSF is a growth factor for erythroid, megakaryocyte, and eosinophil progenitors.\u003cbr\u003e\n500μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: Cynomolgus, Rhesus\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: E. coli-expressed, recombinant human GM-CSF.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.\u003cbr\u003e\nApplication: ELISA Detection, ELISPOT Detection, ICFC\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.25-1.0 µg\/ml is recommended. To obtain a linear standard curve, serial dilutions of human GM-CSF protein ranging from 500 to 4 pg\/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: ELISA1-4 or ELISPOT3,4 Detection, Neutralization1, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated5,6 and acetone-fixed7 frozen tissue sections, and immunocytochemistry.Note: For testing human GM-CSF in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 432001 to 432006) are specially developed and recommended.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Abrams J, et al. 1992. Immunol. Rev. 127:5. (ELISA, Neut, IP) Abrams J, et al. 1994. Eosinophils in Allergy and Inflammation. Marcel Dekker New York. p.133. (ELISA) Bacchetta R, et al. 1990. J. Immunol. 144:902. (ELISA) Kita H, et al. 1991. J. Exp. Med. 174:745. (ELISA) Andersson U, et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag. (IHC) Andersson J, et al. 1994. Immunology 83:16. (IHC) Rasouli J, et al. 2015. J. Immunol. 11:5085-93. (IHC)\u003cbr\u003e\nProduct Citations: Loudon P, et al. 2010. PLoS One. 5:e11021. PubMed Gallo R, et al. 2012. J Immunol. 189:1689. PubMed\u003cbr\u003e\nRRID: AB_315218 (BioLegend Cat. No. 502304)\u003cbr\u003e\nStructure: Cytokine; 22 kD (Mammalian)\u003cbr\u003e\nBioactivity: Growth\/development factor for granulocyte\/macrophage progenitors; differentiates myeloblasts\/monoblasts; synergizes with Epo proliferation of erythroid\/megakaryocytic progenitors\u003cbr\u003e\nCell Sources: T cells, macrophages, fibroblasts, endothelial cells, mast cells\u003cbr\u003e\nCell Targets: Granulocyte\/macrophage\/erythroid\/megakaryocytic progenitors, myeloblasts, monoblasts\u003cbr\u003e\nReceptors: Heterodimer GM-CSFR α subunit (CDw116); β-subunit (CDw131), which is also shared with the IL-3 and IL-5 receptor α chains\u003cbr\u003e\nBiology Area: Cell Biology, Stem Cells\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines, Growth Factors\u003cbr\u003e\nAntigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. Demetri G, et al. 1991. Blood 78:2791. 3. Fan D, et al. 1991. In vivo 5:571. 4. Negrin R, et al. 1992. Adv. Pharmacol. 23:263.\u003cbr\u003e\nRegulation: Synergistic with IL-1, IL-3, G-CSF; E21R competitive antagonist for receptor binding; stored in ECM with heparan sulfate proteoglycans\u003cbr\u003e\nGene ID: 1437\u003cbr\u003e\nUniProt: View information about GM-CSF on UniProt.org\u003cbr\u003e\nClone: BVD2-21C11\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Granulocyte\/macrophage colony-stimulating factor, CSF-α, Pluripoietin-α, Eosinophil colony stimulating factor (Eo-CSF), Burst promoting activity (BPA)\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: How many biotin molecules are per antibody structure?\u003cbr\u003e\nA: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862929723561,"sku":"502304","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-502304","provider":"Iright","version":"1.0","type":"link"}