{"product_id":"biolegend-502602","title":"Biolegend, 502602, Purified anti-human MCP-1 Antibody, 500μg","description":"\u003cp\u003eMonocyte chemotactic protein-1 (MCP-1) also known as monocyte chemotactic and activating factor (MCAF) was identified based on its ability to chemoattract monocytes. Subsequently, MCP-1 has also been found to regulate adhesion molecule expression and cytokine production in monocytes. MCP-1 is identical to the product of the JE gene, a PDGF inducible gene. MCP-1 is a member of the beta (C-C) chemokine subfamily, known as CCL2. The 5D3-F7 antibody reacts with human monocyte chemoattractant protein-1 (MCP-1).\u003cbr\u003e\n500μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: Cynomolgus, Rhesus\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Recombinant human MCP-1\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: ELISA Capture - Quality tested WB - Verified ELISA \u0026amp; ELISPOT Detection, ICFC, IHC, IP - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Recommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 2-8 µg\/ml is recommended. To obtain a linear standard curve, serial dilutions of MCP-1 recombinant protein ranging from 2000 to 31.3 pg\/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: ELISA or ELISPOT Detection1: The biotinylated 5D3-F7 antibody is useful as the detection antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified 2H5 antibody (Cat. No. 505902\/505906) as the capture antibody. ELISA or ELISPOT Capture: The purified 5D3-F7 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 2H5 antibody (Cat. No. 505908) as the detection antibody. The LEAF™ purified antibody (Cat. No. 502607) is suggested for ELISPOT capture. Additional reported applications (for the relevant formats) include: intracellular flow cytometry2, immunoprecipitation1,3, Western blotting1, and immunohistochemical staining1.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Peri, G., et al. 1994. J. Immunol. Meth. 174:249. (IP, IHC, WB) Rezaie-Majd, A., et al. 2002. Arterioscler Thromb Vasc Biol. 22:1194. (ICFC) Hirsch, A., et al. 1999. J. Virol. 73:404. (IP)\u003cbr\u003e\nProduct Citations: Huang HW, et al. 2021. Int J Mol Sci. 22:. PubMed Lima LG, et al. 2021. Nat Commun. 12:3543. PubMed Fang WB, et al. 2021. Sci Rep. 11:8708. PubMed\u003cbr\u003e\nRRID: AB_315240 (BioLegend Cat. No. 502601) AB_315241 (BioLegend Cat. No. 502602)\u003cbr\u003e\nStructure: CC chemokine; dimer; 13.8 kD (Mammalian)\u003cbr\u003e\nBioactivity: Chemoattractant; regulate adhesion molecule expression, cytokine production in monocytes; proliferation\/activation of CC-chemokine activated killer cells\u003cbr\u003e\nCell Sources: Monocytes, vascular endothelial cells, smooth muscle, glomerular mesangial cells\u003cbr\u003e\nCell Targets: Monocytes, basophils, T cells, NK cells, immature dendritic cells\u003cbr\u003e\nReceptors: CCR2; CCR5; CCR10; D6; possibly L-CCR\u003cbr\u003e\nCell Type: Embryonic Stem Cells\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines\u003cbr\u003e\nAntigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. Bischoff S, et al. 1992. J. Exp. Med. 175:1271. 3. Charo I, et al. 1994. P. Natl. Acad. Sci. USA 91:2752. 4. Jiang Y, et al. 1992. J. Immunol. 148:2423.\u003cbr\u003e\nRegulation: Upregulated by IL-1, TNF-α, PDGF, TGF-β\u003cbr\u003e\nGene ID: 6347\u003cbr\u003e\nUniProt: View information about CCL2 on UniProt.org\u003cbr\u003e\nClone: 5D3-F7\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Monocyte chemoattractant protein-1, Monocyte chemoattractant and activating factor (MCAF), JE, Small inducible cytokine A2 (SCYA2), HC-11, P6, Smooth muscle cell chemotactic factor (SMC-CF), CCL2\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864165044393,"sku":"502602","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-502602","provider":"Iright","version":"1.0","type":"link"}