{"product_id":"biolegend-502614","title":"Biolegend, 502614, PE\/Cyanine7 anti-human MCP-1 Antibody, 100tests","description":"\u003cp\u003eMonocyte chemotactic protein-1 (MCP-1) also known as monocyte chemotactic and activating factor (MCAF) was identified based on its ability to chemoattract monocytes. Subsequently, MCP-1 has also been found to regulate adhesion molecule expression and cytokine production in monocytes. MCP-1 is identical to the product of the JE gene, a PDGF inducible gene. MCP-1 is a member of the beta (C-C) chemokine subfamily, known as CCL2. The 5D3-F7 antibody reacts with human monocyte chemoattractant protein-1 (MCP-1).\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nReported Reactivity: Cynomolgus, Rhesus\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Recombinant human MCP-1\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE\/Cyanine7 under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: ELISA or ELISPOT Detection1: The biotinylated 5D3-F7 antibody is useful as the detection antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the purified 2H5 antibody (Cat. No. 505902\/505906) as the capture antibody. ELISA or ELISPOT Capture: The purified 5D3-F7 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 2H5 antibody (Cat. No. 505908) as the detection antibody. The LEAF™ purified antibody (Cat. No. 502607) is suggested for ELISPOT capture. Additional reported applications (for the relevant formats) include: intracellular flow cytometry2, immunoprecipitation1,3, Western blotting1, and immunohistochemical staining1.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Peri, G., et al. 1994. J. Immunol. Meth. 174:249. (IP, IHC, WB) Rezaie-Majd, A., et al. 2002. Arterioscler Thromb Vasc Biol. 22:1194. (ICFC) Hirsch, A., et al. 1999. J. Virol. 73:404. (IP)\u003cbr\u003e\nProduct Citations: Wienke J, et al. 2024. Cancer Cell. 283:42. PubMed Billing F, et al. 2021. ACS Appl Mater Interfaces. 13:55534. PubMed Feuerer N, et al. 2022. Biomedicines. 10:. PubMed\u003cbr\u003e\nRRID: AB_2734490 (BioLegend Cat. No. 502613) AB_2734491 (BioLegend Cat. No. 502614)\u003cbr\u003e\nStructure: CC chemokine; dimer; 13.8 kD (Mammalian)\u003cbr\u003e\nBioactivity: Chemoattractant; regulate adhesion molecule expression, cytokine production in monocytes; proliferation\/activation of CC-chemokine activated killer cells\u003cbr\u003e\nCell Sources: Monocytes, vascular endothelial cells, smooth muscle, glomerular mesangial cells\u003cbr\u003e\nCell Targets: Monocytes, basophils, T cells, NK cells, immature dendritic cells\u003cbr\u003e\nReceptors: CCR2; CCR5; CCR10; D6; possibly L-CCR\u003cbr\u003e\nCell Type: Embryonic Stem Cells\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines\u003cbr\u003e\nAntigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. Bischoff S, et al. 1992. J. Exp. Med. 175:1271. 3. Charo I, et al. 1994. P. Natl. Acad. Sci. USA 91:2752. 4. Jiang Y, et al. 1992. J. Immunol. 148:2423.\u003cbr\u003e\nRegulation: Upregulated by IL-1, TNF-α, PDGF, TGF-β\u003cbr\u003e\nGene ID: 6347\u003cbr\u003e\nUniProt: View information about CCL2 on UniProt.org\u003cbr\u003e\nClone: 5D3-F7\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Monocyte chemoattractant protein-1, Monocyte chemoattractant and activating factor (MCAF), JE, Small inducible cytokine A2 (SCYA2), HC-11, P6, Smooth muscle cell chemotactic factor (SMC-CF), CCL2\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864164978857,"sku":"502614","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-502614","provider":"Iright","version":"1.0","type":"link"}