{"product_id":"biolegend-505910","title":"Biolegend, 505910, APC anti-mouse\/rat\/human MCP-1 Antibody, 100μg","description":"\u003cp\u003eMonocyte chemotactic protein-1 (MCP-1) also known as monocyte chemotactic and activating factor (MCAF) was identified based on its ability to chemoattract monocytes. Subsequently, MCP-1 has also been found to regulate adhesion molecule expression and cytokine production in monocytes. MCP-1 is identical to the product of the JE gene, a PDGF inducible gene. MCP-1 is a member of the beta (C-C) chemokine subfamily, known as CCL2. The 2H5 antibody reacts with mouse, rat, and human MCP-1. The 2H5 antibody can neutralize the bioactivity of natural or recombinant MCP-1.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse, Rat, Human\u003cbr\u003e\nReported Reactivity: Rhesus\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Hamster\u003cbr\u003e\nImmunogen: CHO-expressed, recombinant mouse MCP-1\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: ELISA or ELISPOT Capture1: The Ultra-LEAF™ 2H5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 4E2\/MCP antibody (Cat. No. 506002) as the detecting antibody for the detection of mouse MCP-1. The purified 2H5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 5D3-F7 antibody (Cat. No. 502609) as the detecting antibody for the detection of human MCP-1. Flow Cytometry2: The fluorochrome-labeled 2H5 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify MCP-1 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section. Neutralization1,4,6: The Ultra-LEAF™ purified antibody (Cat. Nos. 505911 \u0026amp; 505912) is recommended for neutralization. Additional reported applications (for the relevant formats) include: Western blotting3, immunohistochemistry of paraformaldehyde-fixed, saponin-treated frozen tissue sections5, and acetone-fixed frozen tissue sections7.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Luo, Y., et al. 1994. J. Immunol. 153:3708. Zhang, Y., et al. 2002. J. Immunol. 168:3088. Luo, Y., et al. 1999 J. Immunol. 163:3985. Morrison, B. E., et al. 2003 J. Clin. Invest. 112:1862. Hancock, W. W., et al. 1997 Transplantation 64:696. Yu, R., et al. 2006 Obesity 14:1353. Taal MW, et al. 2001. Am. J. Physiol. Renal. Physiol. 280:F343. Wan W, et al. 2015. Cardiovasc Res. 106:478. PubMed\u003cbr\u003e\nProduct Citations: Campisi L, et al. 2022. Nature. 606:945. PubMed Jiang P, et al. 2021. Nat Methods. 18:1181. PubMed Pascual–García M, et al. 2019. Nat Commun. 10:2416. PubMed\u003cbr\u003e\nRRID: AB_2566053 (BioLegend Cat. No. 505909) AB_2566054 (BioLegend Cat. No. 505910)\u003cbr\u003e\nStructure: CC chemokine; dimer; 13.8 kD (Mammalian)\u003cbr\u003e\nBioactivity: Chemoattractant; regulates adhesion molecule expression, cytokine production in monocytes; proliferation\/activation of CC-chemokine activated killer cells\u003cbr\u003e\nCell Sources: Monocytes, vascular endothelial cells, smooth muscle, glomerular mesangial cells\u003cbr\u003e\nCell Targets: Monocytes, basophils, T cells, NK cells, immature dendritic cells\u003cbr\u003e\nReceptors: CCR2; CCR5; CCR10; D6; possibly L-CCR\u003cbr\u003e\nBiology Area: Cell Biology, Neuroinflammation, Neuroscience\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines\u003cbr\u003e\nAntigen References: 1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego. 2. Bischoff, S., et al. 1992. J. Exp. Med. 175:1271. 3. Charo, I., et al. 1994. P. Natl. Acad. Sci. USA 91:2752. 4. Jiang, Y., et al. 1992. J. Immunol. 148:2423.\u003cbr\u003e\nRegulation: Upregulated by IL-1, TNF-α, PDGF, TGF-β\u003cbr\u003e\nGene ID: 20296247706347\u003cbr\u003e\nUniProt: View information about CCL2 on UniProt.org\u003cbr\u003e\nClone: 2H5\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Monocyte chemoattractant protein-1, Monocyte chemoattractant and activating factor (MCAF), JE, Small inducible cytokine A2 (SCYA2), HC-11, P6, Smooth muscle cell chemotactic factor (SMC-CF), CCL2\u003cbr\u003e\nIsotype: Hamster IgG\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862327840937,"sku":"505910","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-505910","provider":"Iright","version":"1.0","type":"link"}