{"product_id":"biolegend-506002","title":"Biolegend, 506002, Biotin anti-mouse MCP-1 Antibody, 500μg","description":"\u003cp\u003eMonocyte chemotactic protein-1 (MCP-1) also known as monocyte chemotactic and activating factor (MCAF) was identified based on its ability to chemoattract monocytes. Subsequently, MCP-1 has also been found to regulate adhesion molecule expression and cytokine production in monocytes. MCP-1 is identical to the product of the JE gene, a PDGF inducible gene. MCP-1 is a member of the beta (C-C) chemokine subfamily, known as CCL2. The 4E2\/MCP antibody reacts with mouse MCP-1. This antibody does not react with human MCP-1 and related mouse beta chemokines, TCA3 and MIP-1beta. The 4E2\/MCP antibody can not neutralize the bioactivity of natural or recombinant MCP-1.\u003cbr\u003e\n500μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Hamster\u003cbr\u003e\nImmunogen: CHO-expressed, recombinant mouse MCP-1\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.\u003cbr\u003e\nApplication: ELISA Detection, ELISPOT Detection\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 1-4 µg\/ml is recommended. To obtain a linear standard curve, serial dilutions of MCP-1 recombinant protein ranging from 2000 to 15 pg\/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: ELISA or ELISPOT Capture: The purified 4E2\/MCP antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 2H5 antibody (Cat. No. 505908) as the detecting antibody for the detection of mouse MCP-1. The LEAF™ purified antibody is suggested for ELISPOT capture. Additional reported applications (for the relevant formats) include: Western blotting1. The 4E2\/MCP is a non-neutralizing antibody1.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Luo Y, et al. 1994. J. Immunol. 153:3708. Luo Y, et al. 1999 J. Immunol. 163:3985.\u003cbr\u003e\nProduct Citations: Yang D, et al. 2013. Cereb Cortex. 23:1218. PubMed Feig J, et al. 2011. PLoS One. 6:e28534. PubMed\u003cbr\u003e\nRRID: AB_2281903 (BioLegend Cat. No. 506002)\u003cbr\u003e\nStructure: CC chemokine; dimer; 13.8 kD (Mammalian)\u003cbr\u003e\nBioactivity: Chemoattractant; regulates adhesion molecule expression, cytokine production in monocytes; proliferation\/activation of CC-chemokine activated killer cells\u003cbr\u003e\nCell Sources: Monocytes, vascular endothelial cells, smooth muscle, glomerular mesangial cells\u003cbr\u003e\nCell Targets: Monocytes, basophils, T cells, NK cells, immature dendritic cells\u003cbr\u003e\nReceptors: CCR2; CCR5; CCR10; D6; possibly L-CCR\u003cbr\u003e\nBiology Area: Cell Biology, Neuroinflammation, Neuroscience\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines\u003cbr\u003e\nAntigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego. 2. Bischoff S, et al. 1992. J. Exp. Med. 175:1271. 3. Charo I, et al. 1994. P. Natl. Acad. Sci. USA 91:2752. 4. Jiang Y, et al. 1992. J. Immunol. 148:2423.\u003cbr\u003e\nRegulation: Upregulated by IL-1, TNF-a, PDGF, TGF-ß\u003cbr\u003e\nGene ID: 20296\u003cbr\u003e\nUniProt: View information about MCP-1 on UniProt.org\u003cbr\u003e\nClone: 4E2\/MCP\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Monocyte chemoattractant protein-1, Monocyte chemoattractant and activating factor (MCAF), JE, Small inducible cytokine A2 (SCYA2), HC-11, P6, Smooth muscle cell chemotactic factor (SMC-CF), CCL2\u003cbr\u003e\nIsotype: Hamster IgG\u003cbr\u003e\nQ: How many biotin molecules are per antibody structure?\u003cbr\u003e\nA: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862415069353,"sku":"506002","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-506002","provider":"Iright","version":"1.0","type":"link"}