{"product_id":"biolegend-511802","title":"Biolegend, 511802, Purified anti-mouse IL-12 (p70) Antibody, 500μg","description":"\u003cp\u003eInterleukin 12 (IL-12) is a heterodimeric pleiotropic 70 kD (p70) cytokine consisting of a 40 kD (p40) subunit and a 35 kD (p35) subunit. IL-12 is a potent regulator of cell-mediated immune responses and key mediator of Th1 cell development. Bioactive IL-12 is secreted by activated B lymphocytes, dendritic cells, and macrophages in response to pathogens or upon interaction with activated T cells.IL-12 p70 stimulates cytotoxic T, NK and LAK cell proliferation, andinduces IFN-γ and TNF production in resting and activated T and NK cells.It has been suggested that IL-12 may have therapeutic potential as a vaccine adjuvant that promotes cellular-immunity and as an anti-tumor and anti-viral agent.\u003cbr\u003e\n500μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: CHO expressed, recombinant mouse IL-12 p70\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: ELISA Capture\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA capture antibody, a concentration range of 1-4 µg\/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-12(p70) recombinant protein ranging from 500 to 4 pg\/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: ELISA Capture: The purified C18.2 antibody is useful as a capture antibody for a sandwich ELISA assay, when used in conjunction with the biotinylated C17.8 antibody as detection antibody and recombinant mouse IL-12 p70 as the standard.\u003cbr\u003e\nProduct Citations: Volpedo G, et al. 2022. NPJ Vaccines. 7:32. PubMed Liang D, et al. 2018. Front Immunol. 8:1801. PubMed Zhan CS, et al. 2020. FASEB J. 14006:34. PubMed Jin X, et al. 2020. Cell Reports. 31(8):107690. PubMed Zhou Y, et al. 2018. Cell Mol Immunol. 15:246. PubMed Liu C, et al. 2019. Theranostics. 9:1369. PubMed Zhao L, et al. 2021. STAR Protocols. 2:100732. PubMed\u003cbr\u003e\nRRID: AB_2123771 (BioLegend Cat. No. 511801) AB_2123769 (BioLegend Cat. No. 511802)\u003cbr\u003e\nStructure: heterodimeric cytokine composed of p40 subunit shared with IL-23 and a specific p35 subunit\u003cbr\u003e\nDistribution: Secreted by dendritic cells, monocytes\/macrophages, B cells, T cells\u003cbr\u003e\nFunction: Downregulated by IL-10; homodimeric p40 antagonistic to functional p70 heterodimer; p40 monomer has no function; p40 subunit in common with IL-23\u003cbr\u003e\nInteraction: T cells, NK cells\u003cbr\u003e\nLigand\/Receptor: IL-12Rβ1 binds p40 shared with IL-23; dimeric with IL-12Rβ2 binds p35\u003cbr\u003e\nBioactivity: IL-12 p70 (p40:p35) induces IFN-γ, TNF-α production in T and NK cells; costimulation of PBL proliferation; proliferation\/differentiation of TH1 T lymphocytes.\u003cbr\u003e\nCell Type: Dendritic cells, Monocytes, Macrophages, B cells, T cells\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines\u003cbr\u003e\nAntigen References: 1. Lankford CS, et al. 2003 J. Leukoc. Biol.. 73:49.2. Parham C, et al. 2002 J. Immunol.. 168:5699.3. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.4. Trinchieri G, et al. 1993 Immunol. Today. 14:335.5. Quesniaux V. 1992. Research Immunol. 143:385.6. Trinchieri G, et al. 1992 Prog. Growth Factor Res. 4:355.\u003cbr\u003e\nGene ID: 1615916160\u003cbr\u003e\nUniProt: View information about IL-12 p70 on UniProt.org\u003cbr\u003e\nClone: C18.2\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Interleukin-12 p70, Cytotoxic lymphocyte maturation factor (CLMF), Natural killer cell stimulatory factor (NKSF)\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862940537001,"sku":"511802","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-511802","provider":"Iright","version":"1.0","type":"link"}