{"product_id":"biolegend-512201","title":"Biolegend, 512201, Purified anti-human TSLP Antibody, 50μg","description":"\u003cp\u003eThymic Stromal Lymphopoietin (TSLP) is a hemopoietic protein that is expressed in the heart, liver and prostate. TSLP overlaps biological activities with IL-7 and binds with the heterodimeric receptor complex consisting of the IL-7R alpha chain (IL-7Rα) and the TSLP-specific chain (TSLPR). Like IL-7, TSLP induces phosphorylation of STAT3 and STAT5, but uses kinases other than the JAKs for activation. TSLP prohibited apoptosis and stimulated growth of the human acute myeloid leukemia (AML)-derived cell line MUTZ3. It induces the release of T cell-attracting chemokines TARC and MDC from monocytes and activates CD11c(+) dendritic cells (DCs). TSLP activated DCs primed naïve T cells to produce the proallergic cytokines (IL-4, IL-5, IL-13, TNFα) while down-regulating IL-10 and IFN-γ suggesting a role in initiating allergic inflammation. Recent studies revealed that expression of TSLP has a direct link to the pathogenesis of allergic inflammation such as atopic dermatitis and asthma via activating dendritic cells and mast cells which trigger inflammatory Th2 response characterized by high TNF-alpha and little IL-10 production.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Recombinant human TSLP\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: ELISA Capture - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA capture antibody, a concentration range of 1-4 µg\/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application. To obtain a linear standard curve, serial dilutions of TSLP recombinant protein ranging from 200 to 3.1 ng\/ml are recommended for each ELISA plate.\u003cbr\u003e\nApplication Notes: ELISA Capture: Purified and Ultra-LEAF™ purified 15B11.3 antibody are useful as a capture antibody for a sandwich ELISA assay, when used in conjunction with the biotinylated Poly5159 antibody (Cat. No. 515903) as the detecting antibody.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Yeh CY, et al. 2013. PLoS One. 8:e55472. (Neut) PubMed\u003cbr\u003e\nRRID: AB_940352 (BioLegend Cat. No. 512202)\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines\u003cbr\u003e\nAntigen References: 1. Liu YJ. 2007. J. Allergy Clin. Immunol. 120:238. 2. Nagata Y. 2007. Int. Arch. Allergy Immunol. 144:305. 3. Jiang Q, et al. 2007 BMC Immunol. 8:11. 4. Kato A. et al. 2007. J. Immunol. 179:1080. 5. Rochman I, et al. 2007 J. Immunol. 178:6720. 6. Reche PA, et al. 2001. J. Immunol. 167:336. 7. Pandey A, et al. 2000. Nat. Immunol. 1:59. 8. Park LS, et al. 2000. J Exp. Med. 192:659.\u003cbr\u003e\nGene ID: 85480\u003cbr\u003e\nUniProt: View information about TSLP on UniProt.org\u003cbr\u003e\nClone: 15B11.3\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Thymic stromal lymphopoietin (TSLP)\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860811763881,"sku":"512201","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-512201","provider":"Iright","version":"1.0","type":"link"}