{"product_id":"biolegend-516301","title":"Biolegend, 516301, Purified anti-human IL-33 Antibody, 100μg","description":"\u003cp\u003eIL-33, also known as NFHEV and DVS 27, is a 30 kD proinflammatory protein that belongs to IL-1 superfamily. IL-33 is constitutively expressed in smooth muscle and airway epithelia. It is upregulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL-1α or IL-1β stimulation. IL-33 interacts with IL1RL1\/ST2 receptor and IL-1 receptor accessory protein (IL1RAP) in order to activate intracellular molecules in the NF-κB and MAP kinase signaling pathways that drive production of type 2 cytokines (e.g. IL-5 and IL-13) from polarized Th2 cells. Recombinant IL-33 has been shown to enhance Th2-associated immune responses and potently increase mast cell proliferation and cytokine production.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Polyclonal\u003cbr\u003e\nHost Species: Goat\u003cbr\u003e\nImmunogen: Human IL-33, amino acids Ser112-Thr270 (Accession# NM_ 033439) was expressed in E. coli.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by antigen affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.\u003cbr\u003e\nApplication: ELISA Capture - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA capture antibody, a concentration range of 0.25-1 µg\/ml is recommended. It is recommended that the reagent be titrated for optimal performance. To obtain a linear standard curve, serial dilutions of IL-33 recombinant protein ranging from 1000-15.6 pg\/ml are recommended.\u003cbr\u003e\nApplication Notes: ELISA Capture: The purified Poly5163 antibody is useful as a capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated Poly5163 antibody (Cat. No. 516303) as the detecting antibody and recombinant human IL-33 (Cat. No. 581809) as the standard.\u003cbr\u003e\nRRID: AB_2561331 (BioLegend Cat. No. 516301)\u003cbr\u003e\nStructure: 270 aa with predicted molecular weight of 30 kD.\u003cbr\u003e\nDistribution: Full length IL-33 localizes to the nucleus in HUVECs and transfectants C-terminal fragment, corresponding mature IL-33 is secreted.\u003cbr\u003e\nFunction: Cytokine that binds to and signals through IL1RL1\/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3\/ERK1 and\/or MAPK1\/ERK2, MAPK14, and MAPK8. Induces T helper type 2-associated cytokines.\u003cbr\u003e\nInteraction: Binding to IL1RL1\/ST2 and IL-1 receptor accessory protein.\u003cbr\u003e\nCross: In sandwich ELISA, no cross-reactivity with mouse or rat IL-33.\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines\u003cbr\u003e\nAntigen References: 1. Lohning M, et al. 1998. P. Natl. Acad. Sci. USA 95:693. 2. Onda H, et al. 1999. J. Cerebr. Blood F. Met. 19:1279. 3. Schmitz J, et al. 2005. Immunity 23:479. 4. McLaren JE, et al. 2010. J. Immunol. 185:1222.\u003cbr\u003e\nGene ID: 90865\u003cbr\u003e\nUniProt: View information about IL-33 on UniProt.org\u003cbr\u003e\nClone: Poly5163\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Interleukin-33\u003cbr\u003e\nIsotype: Goat Polyclonal Ig\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866163269801,"sku":"516301","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-516301","provider":"Iright","version":"1.0","type":"link"}