{"product_id":"biolegend-532304","title":"Biolegend, 532304, Biotin anti-mouse NGAL (Lipocalin-2) Antibody, 100μg","description":"\u003cp\u003eNGAL (lipocalin-2) is a lipocalin family protein that plays important roles in innate immunity, inflammation, and atherosclerosis. Mouse NGAL is detected in lung, spleen, uterus, vagina, and epididymis. It is a critical component of innate immunity because of its ability to bind iron-siderophore complexes and limit bacterial growth. It is also produced by injured epithelia and is one of the most promising new markers for renal epithelial injury and cardiovascular disease (CVD).\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: In-house recombinant mouse NGAL\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.\u003cbr\u003e\nApplication: ELISA Detection - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by ELISA. For ELISA detection applications, a concentration of 0.1 µg\/mL is recommended. To obtain a linear standard curve, serial dilutions of NGAL recombinant protein ranging from 1000 to 15.6 pg\/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance.\u003cbr\u003e\nApplication Notes: The biotinylated M0417A8 antibody is useful as a detection antibody for a sandwich ELISA assay, when used in conjunction with purified M047A10 (Cat. No. 532202) antibody as the capture antibody. Note: For testing mouse NGAL in cell culture supernatant, serum, plasma, urine and other biological fluids, BioLegend's LEGEND MAX™ Kits (Cat. No. 443707 \u0026amp; 443708) are specially developed and recommended.\u003cbr\u003e\nRRID: AB_2686952 (BioLegend Cat. No. 532304)\u003cbr\u003e\nStructure: 195 amino acid monomer with a predicted molecular weight of approximately 23 kD.\u003cbr\u003e\nDistribution: Secreted by epithelial cells, macrophages, neutrophils; increased levels have been observed in plasma, serum, and urine in various conditions, such as metastatic breast and colorectal cancer, acute kidney injury, pancreatitis and preeclampsia.\u003cbr\u003e\nFunction: Apoptosis, innate immunity, renal development.\u003cbr\u003e\nInteraction: Disulfide-linked with MMP9.\u003cbr\u003e\nLigand\/Receptor: SLC22A17 (24p3R)\u003cbr\u003e\nCell Type: Epithelial cells, Macrophages, Neutrophils\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cell Biology, Immunology, Innate Immunity, Signal Transduction\u003cbr\u003e\nAntigen References: 1. Iqbal N, et al. 2013. Expert Opin. Med Diagn. 7:209. 2. Singer E, et al. 2013. Acta. Physiol. (Oxf) 207:663. 3. Palazzuoli A, et al. 2012. Clin. Chim. Acta. 413:1832. 4. Flo TH, et al. 2004. Nature 432:917. 5. Goetz DH, et al. 2002. Mol. Cell. 10:1033. 6. Leung L, et al. 2012. PLoS One 7:e46677. 7. Kjeldsen L, et al. 1993. J. Biol. Chem. 268:10425.\u003cbr\u003e\nGene ID: 16819\u003cbr\u003e\nUniProt: View information about NGAL on UniProt.org\u003cbr\u003e\nClone: M0417A8\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Lipocalin 2, Oncogene 24p3, Neutrophil Gelatinase-Associated Lipocalin, Siderocalin LCN2, 25 kD Alpha-2-Microglobulin-Related Subunit Of MMP-9, p25, MSFI, Migration-Stimulating Factor Inhibitor, HNL\u003cbr\u003e\nIsotype: Rat IgG2a, λ\u003cbr\u003e\nQ: How many biotin molecules are per antibody structure?\u003cbr\u003e\nA: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866123325609,"sku":"532304","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-532304","provider":"Iright","version":"1.0","type":"link"}