{"product_id":"biolegend-537102","title":"Biolegend, 537102, Purified anti-human IFN-α2 Antibody, 500μg","description":"\u003cp\u003eInterferons are divided into type I, II, and III. Type I IFNs (IFN-α and IFN-β) are most abundant in number, distribution, and expression. Also, they are highly conserved among mammals in both structure and function. IFN-α2 has been used in the treatment of cancer such as bladder cancer, hepatocellular carcinoma, and leukemia. IFN-α2 augments the suppressed immune functions in patients with head and neck squamous cell carcinoma (HNSCC). IFN-α2 initiated T and NK cell mediated cytotoxicity of tumor cells through IFNγ dependent and independent mechanisms. IFN-α2 enhances suppressed T cell cytotoxicity by stimulation of the perforin-granzyme B system (IFNγ dependent). Also, IFN-α2 induces the expression of perforin-granzyme B in NK cells (NK mediated cytotoxicity, IFNγ independent). In a preliminary study, IFN-α2 appears to be an effective immunostimulator and impacts the clinical outcome in tongue squamous cell carcinoma patients. IFN-α had been used in the treatment of chronic hepatitis C (CHC); nevertheless, IFN-α is relatively unstable and requires frequent parenteral administration. Pegylation of IFN-α, polyethylene glycol (PEG)-IFN-α, reduces in vitro activity but increase the stability and plasma half-life of IFN-α; therefore, PEG-IFN-α has replaced IFN-α in CHC treatment.\u003cbr\u003e\n500μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Recombinant protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: ELISA Capture - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by ELISA assay. For ELISA Capture applications, a concentration range of 2.0 - 8.0 µg\/mL is recommended. To obtain a linear standard curve, serial dilutions of IFN-α2 recombinant protein ranging from 12.5 to 800 pg\/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: The pure M4509E12 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated BL25372 antibody (Cat. No. 537204) as the capture antibody and Recombinant Human IFN-a2 (ELISA Std.) (Cat. No. 592709)\u003cbr\u003e\nRRID: AB_2783346 (BioLegend Cat. No. 537102)\u003cbr\u003e\nDistribution: IFN-α is ubiquitously expressed.\u003cbr\u003e\nFunction: IFN-α stimulates cytotoxic T lymphocytes and natural killer cell function. IFN-α has immunomodulatory and antiviral properties. IFNα2a induces CXCL10 in human microvascular endothelial cells and pulmonary artery smooth muscle cells. It is induced by viral infection.\u003cbr\u003e\nInteraction: A variety of cells express the IFNα receptor (IFNAR).\u003cbr\u003e\nLigand\/Receptor: IFNAR complex has two components, IFNAR1 and IFNAR2.\u003cbr\u003e\nBiology Area: Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: CD Molecules, Soluble Receptors\u003cbr\u003e\nAntigen References: Bose A and Baral R. 2007. Immunol Lett. 108:68. Zhao W, et al. 2008. J Immunol. 180:5483. Badiger R, et al. 2012. Plos One. 7:e46779. Aghemo A, et al. 2010. Nat Rev Gastroenterol Hepatol. 7:485. Becker-Merok A, et al. 2013. Lupus. 22:155. Mukherjee KK, et al. 2012. Indian J Med Res. 136:54.\u003cbr\u003e\nGene ID: 3440\u003cbr\u003e\nUniProt: View information about IFN-alpha2 on UniProt.org\u003cbr\u003e\nClone: M4509E12\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: IFN-alpha 2B, IFN-alphaA, IFNA2, IFNA2B, Interferon Alpha 2\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866124144809,"sku":"537102","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-537102","provider":"Iright","version":"1.0","type":"link"}