{"product_id":"biolegend-568104","title":"Biolegend, 568104, Biotinylated Recombinant Human CD38 (carrier-free), 25μg","description":"\u003cp\u003eCD38 is a 45 kD type II transmembrane glycoprotein also known as T10. It is an ADP-ribosyl hydrolase expressed at variable levels on hematopoietic cells and in some non-hematopoietic tissues (such as brain, muscle, and kidney). In humans, it is expressed at high levels on plasma cells and activated T and B cells, natural killer (NK) lymphocytes, myeloblasts, and erythroblasts. By functioning as both a cyclase and a hydrolase, CD38 mediates lymphocyte activation, adhesion, and the metabolism of cADPR and NAADP. CD31 is the ligand of CD38. ADP-ribosyl cyclase is able to transform NAD(P)+ into three different products with calcium mobilizing ability, cyclic ADP-ribose, NAADP+, and ADP-ribose.\u003cbr\u003e\n25μg\u003cbr\u003e\nSource: Biotinylated recombinant human CD38, amino acid Val 43 - Ile 300 (Accession No.NP_001766.2), with a C-terminal Avi-6His tag, was expressed in CHO cells. Human CD38-Avi tag was site-specifically biotinylated by enzyme BirA.\u003cbr\u003e\nMolecular Mass: The 294 amino acid recombinant protein has a predicted molecular mass of approximately 33.5 kD. The DTT-reduced and non-reduced protein migrates at approximately 35 – 50 kD by SDS-PAGE. The predicted N-terminal amino acid is Val.\u003cbr\u003e\nPurity: \u0026gt; 95%, as determined by Coomassie stained SDS-PAGE.\u003cbr\u003e\nFormulation: 0.22 µm filtered protein solution is in PBS, pH7.2.\u003cbr\u003e\nEndotoxin Level: Less than 0.1 EU per µg of protein as determined by the LAL method.\u003cbr\u003e\nConcentration: 25 µg size is bottled at 200 µg\/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.\u003cbr\u003e\nStorage \u0026amp; Handling: Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin the vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg\/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze\/thaw cycles.\u003cbr\u003e\nActivity: Immobilized purified anti-human CD38 antibody at 1 µg\/mL binds recombinant human CD38 in a dose-dependent manner. HRP avidin (Cat. No. 405103) was used to detect the binding. The ED50 for this effect is 0.7 – 4.2 ng\/mL.\u003cbr\u003e\nApplication: Bioassay\u003cbr\u003e\nApplication Notes: BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at [email protected].\u003cbr\u003e\nStructure: ADP-ribosyl cyclase, ectoenzyme, type II glycoprotein, 45 kD\u003cbr\u003e\nDistribution: T cells, B cells, NK-cells, myeloid, plasma, and dendritic cells\u003cbr\u003e\nFunction: Ecto-ADP-ribosyl cyclase, calcium signaling, cell activation\u003cbr\u003e\nInteraction: NK cells, Neutrophils, Macrophages\u003cbr\u003e\nLigand\/Receptor: CD31, hyaluronic acid\u003cbr\u003e\nBioactivity: Measured by its ability to bind CD38 antibody\u003cbr\u003e\nCell Type: B cells, Dendritic cells, NK cells, Plasma cells, T cells\u003cbr\u003e\nBiology Area: Cell Adhesion, Cell Biology, Immunology\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: Gunawan M, et al. 2017. Sci Rep. 7:16642. Martin JC, et al. 2020. Cell. 178:1493. Del Alcazar D, et al 2019. Cell Rep. 28:3047-3060.e7. Kennedy-Darling J, et al. 2021. Eur J Immunol. 51:1262-1277. Cole C, et al. 2018. Nucleic Acids Res. 46:e62. Lavin Y, et al. 2017. Cell. 169:750-765.\u003cbr\u003e\nGene ID: 952\u003cbr\u003e\nUniProt: View information about CD38 on UniProt.org\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: CD38, T10, CD38 antigen (p45), CD38 antigen, CD38 molecule, cluster of differentiation 38, Cyclic ADP-ribose hydrolase 1, CyclicADP-ribose Hydrolase, NAD(+) nucleosidase\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864221601961,"sku":"568104","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-568104","provider":"Iright","version":"1.0","type":"link"}