{"product_id":"biolegend-600452","title":"Biolegend, 600452, Purified anti-JunB Antibody, 100μg","description":"\u003cp\u003eJunB is a member of the AP-1 family of transcription factors, which also includes c-Jun, c-Fos, Fra1, and JunD. JunB can form dimers with other Jun and Fos proteins, as well as members of the ATF transcription factor family, to form a functional AP-1 transcription factor complex. AP-1 complexes containing JunB can exert both activator and repressor function depending on the interacting partner and promoter sequence context. JunB displays both tumor suppressor and oncogenic activity, inducing genes involved in cell senescence and repressing genes involved in proliferation. JunB is required for T helper type 2 (Th2), T helper 17 (Th17) cell and regulatory T cell (Treg) differentiation. Multiple cancers display aberrant JunB expression and function. In some lymphomas, notably Hodgkin’s lymphoma, JunB expression is elevated and the protein functions as an oncogenic transcription factor. Conversely, loss of JunB is observed in chronic myeloid leukemia.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Partial recombinant human JunB protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: ICC - Quality tested ICFC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 5.0 μg\/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nAdditional Product Notes: When using this clone for ICC, we recommend 4% PFA + Triton X-100 for fixation and permeabilization. Other methods tested (4% plus methanol and methanol only) fixation and permeabilization methods produced high non-specific staining.\u003cbr\u003e\nRRID: AB_2910470 (BioLegend Cat. No. 600451) AB_2910470 (BioLegend Cat. No. 600452)\u003cbr\u003e\nStructure: JunB is a 347 amino acid protein with a predicted molecular weight of 36 kD.\u003cbr\u003e\nDistribution: Nucleus\u003cbr\u003e\nFunction: Transcription Factor\u003cbr\u003e\nBiology Area: Cell Biology, Cell Cycle\/DNA Replication, Cell Proliferation and Viability, Transcription Factors\u003cbr\u003e\nAntigen References: Shaulian E and Karin M. 2002. Nat Cell Biol. 4:E131-6. Yang MY, et al. 2003. Blood. 101:3205-11. Carr T, et al. 2017. Nat Commun. 8:301. Katagiri T, et al. 2019. Mucosal Immunol. 12:1104-1117.\u003cbr\u003e\nGene ID: 3726\u003cbr\u003e\nUniProt: View information about JunB on UniProt.org\u003cbr\u003e\nClone: W20103F\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Activator Protein 1, AP-1\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866421350569,"sku":"600452","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-600452","provider":"Iright","version":"1.0","type":"link"}