{"product_id":"biolegend-600753","title":"Biolegend, 600753, Alexa Fluor® 488 anti-c-Fos Antibody, 25tests","description":"\u003cp\u003eThe c-Fos protein, or FBJ murine viral (v-Fos) oncogene homolog, is a member of the Fos family of transcription factors. C-Fos forms heterodimers with proteins of the Jun family of transcription factors to form the AP-1 (Activator Protein-1) complex, which regulates critical cellular processes, including cell proliferation, differentiation, and survival. C-Fos has been demonstrated to play a critical role in angiogenesis, bone development, and in the progression of cancer. C-Fos is also often used as an indirect marker of neuronal activity.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Purified recombinant human FOS protein fragments\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: For ICC we recommend PFA-MeoH fixation\/permeabilization. We do not recommend methanol-only and PFA+ Triton X-100 fixation\/permeabilization due to dim signal. For ICFC, we recommend fixation\/permeabilization with the True-Phos™ Perm Buffer. Cytokine method was tried and failed due to no signal.\u003cbr\u003e\nRRID: AB_3097468 (BioLegend Cat. No. 600753) AB_3097468 (BioLegend Cat. No. 600754)\u003cbr\u003e\nStructure: c-Fos is a 330 amino acid protein with a predicted molecular weight of 41 kD.\u003cbr\u003e\nDistribution: Expressed in the nucleus of many tissues\/cells including placenta, brain, thyroid, central nervous system, lung, endometrium, and B lymphocytes\u003cbr\u003e\nFunction: FOS and JUN oncoproteins form dimeric complexes that stimulate transcription of genes containing AP-1 regulatory elements. FOS was the first transcription factor identified to have a critical function in the development of cells required for skeletal formation.\u003cbr\u003e\nInteraction: Interacts with a variety of proteins including c-Jun, TFIID, NCOA2, NFAT1, CHOP, ELK1, SRC1, and ETS1\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cancer Biomarkers, Cell Biology, Cell Cycle\/DNA Replication, Cell Death, Cell Proliferation and Viability, Transcription Factors\u003cbr\u003e\nAntigen References: Dony C and Gruss P. 1987. Nature. 328:711-4. Grigoriadis AE, et al. 1994. Science. 266:443-8. Glover JN and Harrison SC. 1995. Nature. 373:257-61.\u003cbr\u003e\nGene ID: 2353\u003cbr\u003e\nUniProt: View information about c-Fos on UniProt.org\u003cbr\u003e\nClone: 7D6-A9-D4\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Oncogene Fos, FBJ murine viral (v-fos) oncogene homolog, G0\/G1 Switch Regulatory Protein 7, Activator Protein 1, GOS7, cfos\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862487191721,"sku":"600753","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-600753","provider":"Iright","version":"1.0","type":"link"}