{"product_id":"biolegend-600854","title":"Biolegend, 600854, Alexa Fluor® 647 anti-FosB Antibody, 100tests","description":"\u003cp\u003eFosB is a member of the FOS family of proteins that dimerizes with proteins of the Jun family to form the AP-1 transcription factor complex. As part of AP-1, FosB plays a critical role in regulating cell differentiation, proliferation, and apoptosis. FosB is expressed in most tissues and has been particularly well-studied in the CNS. ΔFosB, a truncated splice variant of the FOSB gene, plays a critical role in the brain’s reward circuit plasticity, with significant impact on both natural and pathological behavior. FosB also functions as a tumor suppressor, and dysregulation of FosB activity or levels has been found in numerous cancers, including gastric, prostate and colorectal cancer.\u003cbr\u003e\n100tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Full-length recombinant FosB protein\u003cbr\u003e\nFormulation: µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.\u003cbr\u003e\nConcentration: µg sizes: 0.5 mg\/mLtest sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC (test sizes) - Quality tested ICC (µg size) - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of the test-sized antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Each lot of the µg-sized antibody is quality control tested by immunocytochemistry. For immunocytochemistry using the ug size, a concentration range of 1.25 - 5.0 μg\/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm \/ 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: Methanol only and PFA + Methanol fix\/perm methods were tested and produced inferior signal. ICFC: Cells were fixed and permeabilizes using True Phos Buffer system ICC: Cells were fixed with 4% PFA and permeabilized with Triton X-100\u003cbr\u003e\nRRID: AB_2941600 (BioLegend Cat. No. 600853) AB_2941600 (BioLegend Cat. No. 600856) AB_2941600 (BioLegend Cat. No. 600854)\u003cbr\u003e\nStructure: FosB is a 338 amino acid protein with a predicted molecular weight of 36kD.\u003cbr\u003e\nDistribution: FosB is widely expressed in developing and adult tissues, including the brain, thyroid, bladder and adipose tissue.\u003cbr\u003e\nFunction: FosB heterodimerizes with proteins of the Jun family to form the transcription factor AP-1 to regulate cell proliferation, survival, and differentiation.\u003cbr\u003e\nInteraction: Jun family proteins e.g. c-Jun, genes with the TPA response element (TGA G\/C TCA) SWI\/SNF chromatin remodeling complex, DPP4\u003cbr\u003e\nCell Type: Mature Neurons\u003cbr\u003e\nBiology Area: Cell Biology, Cell Proliferation and Viability, Neuroscience, Neuroscience Cell Markers, Transcription Factors\u003cbr\u003e\nMolecular Family: Tumor Suppressors\u003cbr\u003e\nAntigen References: Nestler E. 2015. Eur J Pharmacol. 753:66-72. Milde-Langosch K. 2005. Eur J Cancer. 41:2449-61.\u003cbr\u003e\nGene ID: 2354\u003cbr\u003e\nUniProt: View information about FosB on UniProt.org\u003cbr\u003e\nClone: W19011B\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: G0\/G1 switch regulatory protein 3 (G0S3), FosB Proto-Oncogene, AP-1 Transcription Factor Subunit, Oncogene Fos-B, FBJ Murine Osteosarcoma Viral Oncogene Homolog B\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866341724329,"sku":"600854","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-600854","provider":"Iright","version":"1.0","type":"link"}