{"product_id":"biolegend-600857","title":"Biolegend, 600857, PE anti-FosB Antibody, 25tests","description":"\u003cp\u003eFosB is a member of the FOS family of proteins that dimerizes with proteins of the Jun family to form the AP-1 transcription factor complex. As part of AP-1, FosB plays a critical role in regulating cell differentiation, proliferation, and apoptosis. FosB is expressed in most tissues and has been particularly well-studied in the CNS. ΔFosB, a truncated splice variant of the FOSB gene, plays a critical role in the brain’s reward circuit plasticity, with significant impact on both natural and pathological behavior. FosB also functions as a tumor suppressor, and dysregulation of FosB activity or levels has been found in numerous cancers, including gastric, prostate and colorectal cancer.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Full-length recombinant FosB protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: For ICC, we recommend fixation in 4% PFA and permeabilization for 6 minutes in 0.5% Triton-X. Methanol only and PFA + Methanol fix\/perm methods were tested and produced inferior signal. ICFC: Cells were fixed and permeabilized using True Phos Buffer system.\u003cbr\u003e\nRRID: AB_2941601 (BioLegend Cat. No. 600857) AB_2941601 (BioLegend Cat. No. 600858)\u003cbr\u003e\nStructure: FosB is a 338 amino acid protein with a predicted molecular weight of 36kD.\u003cbr\u003e\nDistribution: FosB is widely expressed in developing and adult tissues, including the brain, thyroid, bladder and adipose tissue.\u003cbr\u003e\nFunction: FosB heterodimerizes with proteins of the Jun family to form the transcription factor AP-1 to regulate cell proliferation, survival, and differentiation.\u003cbr\u003e\nInteraction: Jun family proteins e.g. c-Jun, genes with the TPA response element (TGA G\/C TCA) SWI\/SNF chromatin remodeling complex, DPP4\u003cbr\u003e\nCell Type: Mature Neurons\u003cbr\u003e\nBiology Area: Cell Biology, Cell Proliferation and Viability, Neuroscience, Neuroscience Cell Markers, Transcription Factors\u003cbr\u003e\nMolecular Family: Tumor Suppressors\u003cbr\u003e\nAntigen References: Nestler E. 2015. Eur J Pharmacol. 753:66-72. Milde-Langosch K. 2005. Eur J Cancer. 41:2449-61.\u003cbr\u003e\nGene ID: 2354\u003cbr\u003e\nUniProt: View information about FosB on UniProt.org\u003cbr\u003e\nClone: W19011B\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: G0\/G1 switch regulatory protein 3 (G0S3), FosB Proto-Oncogene, AP-1 Transcription Factor Subunit, Oncogene Fos-B, FBJ Murine Osteosarcoma Viral Oncogene Homolog B\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866341789865,"sku":"600857","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-600857","provider":"Iright","version":"1.0","type":"link"}