{"product_id":"biolegend-603552","title":"Biolegend, 603552, Purified anti-OGT Antibody, 100μg","description":"\u003cp\u003eUDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit (OGT) catalyzes the transfer of N-acetyl glucosamine (GlcNAc) post-translational modification to serine, threonine, and cysteine residues of proteins. Serine and threonine residues are modified by O-glycosidic linkage, while cysteine is modified by S-glycosidic linkage. Since the amino acids utilized for O-GlcNAcylation are identical to those used for protein phosphorylation, OGT competes with kinases for modification of those sites. Numerous functions for GlcNAcylation have been reported in regulating protein-protein interactions, and OGT plays diverse roles in the regulation of the cell cycle, transcription, and cellular stress. Dysregulation of O-GlcNAcylation plays a role in the pathology of Alzheimer’s disease, cancer and diabetes. OGT activity is regulated by glucose availability, and plays a role in regulating glycolysis by glycosylation of phosphofructokinase.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Recombinant human OGT protein fragments\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1 µg\/mL. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: This product does not meet passing criteria for ICC and IHC.\u003cbr\u003e\nRRID: AB_2922635 (BioLegend Cat. No. 603551) AB_2922635 (BioLegend Cat. No. 603552)\u003cbr\u003e\nStructure: OGT is a 1,046 amino acid protein with a predicted molecular mass of 116.9 kD.\u003cbr\u003e\nDistribution: OGT is expressed in the cytoplasm of most cells.\u003cbr\u003e\nFunction: OGT is an enzyme which catalyzes the attachment of the O-GlcNAc post-translational modification.\u003cbr\u003e\nInteraction: OGT glycoylates a wide array of proteins including histone H2B, AKT1, and EZH2.\u003cbr\u003e\nBiology Area: Cell Cycle\/DNA Replication, Cell Proliferation and Viability, Protein Synthesis\u003cbr\u003e\nMolecular Family: Enzymes and Regulators, Protein Modifiers\u003cbr\u003e\nAntigen References: Canovas B and Nebreda A. 2021. Nat Rev Mol Cell Biol. 22:346-366.\u003cbr\u003e\nGene ID: 8473\u003cbr\u003e\nUniProt: View information about OGT on UniProt.org\u003cbr\u003e\nClone: W20094A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: O-linked N-acetylglucosaminyltransferase (O-GlcNAc transferase, OGT) subunit p110, UDP-N-acetylglocuosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866420990121,"sku":"603552","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-603552","provider":"Iright","version":"1.0","type":"link"}