{"product_id":"biolegend-604652","title":"Biolegend, 604652, Purified anti-GSK3β Antibody, 100μg","description":"\u003cp\u003eGSK3B is a serine-threonine kinase and a member of the glycogen synthase kinase subfamily. It is a negative regulator of the hormonal control of glucose, Wnt signaling and also transcriptional factors and microtubules. It has been associated with regulation of several cellular mechanisms such as glycogen synthesis, protein synthesis, cell proliferation, microtubules stabilization, cell migration, autophagy and apoptosis. GSK3B is activated by phosphorylation at tyrosine 216 and inactivated by phosphorylation at serine 9. Defects on GSK3B has been associated with Alzheimer’s and Parkinson’s diseases.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human, Rat, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Synthetic peptide conjugated to KLH\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: ICC - Quality tested IHC-P - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg\/mL is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 4.0 - 6.0 µg\/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: For ICC, we recommend 4% PFA fixation followed by permeabilization with ice-cold methanol, or fixation\/permeabilization with ice cold methanol alone. We do not recommend 4% PFA fixation followed by permeabilization with 0.5% Triton-X. For IHC-P, we recommend antigen retrieval using citrate buffer, HIER, and overnight antibody incubation at 4°C.\u003cbr\u003e\nRRID: AB_2922641 (BioLegend Cat. No. 604651) AB_2922641 (BioLegend Cat. No. 604652)\u003cbr\u003e\nStructure: GSK3B is a 420 amino acid protein with a mass of 46.7 kD\u003cbr\u003e\nDistribution: GSK3B is expressed in the cytoplasm of testis, prostate, ovary and at lower levels in lung, brain and kidney\u003cbr\u003e\nFunction: GSK3B is a kinase involved in the regulation of several signaling pathways involving transcriptional factors, hormonal control of glucose, microtubule rearrangement, cell migration and proliferation, apoptosis and autophagy.\u003cbr\u003e\nBiology Area: Cell Biology, Cell Proliferation and Viability, Protein Synthesis\u003cbr\u003e\nMolecular Family: Protein Kinases\/Phosphatase\u003cbr\u003e\nAntigen References: Wu H, et al. 2019. Autophagy. 15:1506-1522. Edderkaoui M, et al. 2018. Gastroenterology. 155:1985-1998. Ryu HY, et al. 2021. Exp Mol Med. 53:369-383. Myöhänen TT, et al. 2021. Basic Clin Pharmacol Toxicol. 129:287-296. Taylan E, et al. 2020. Gynecol Oncol. 159:277-284. Mancinelli R, et al. 2017. Oxid Med Cell Longev. 2017:4629495.\u003cbr\u003e\nGene ID: 2932\u003cbr\u003e\nUniProt: View information about GSK3beta on UniProt.org\u003cbr\u003e\nClone: 9B1-5C3-5G8\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: glycogen synthase kinase 3 beta, Serine\/Threonine-Protein Kinase GSK3B, Glycogen Synthase Kinase-3 Beta\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862502822057,"sku":"604652","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-604652","provider":"Iright","version":"1.0","type":"link"}