{"product_id":"biolegend-606451","title":"Biolegend, 606451, Purified anti-SMAD2 Antibody, 25μg","description":"\u003cp\u003eSMAD2 is a potent regulator of different cellular processes such as apoptosis, differentiation, and cell proliferation. SMAD2 is associated with SARA protein and the TGF-B receptor. SMAD2 gets phosphorylated by the TGF-B receptor in response to TGF-B signal, which induces its dissociation from SARA and association with SMAD4 followed by translocation into the nucleus. In the nucleus, the SMAD2\/SMAD4 protein participates in gene regulation by forming a transcription repressor complex in association with other cofactors.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Recombinant fragments of human SMAD2\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 1.0 µg\/mL. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Since SMAD2 shares a high percentage of identity with SMAD3, this clone recognizes both SMAD2 and SMAD3, which can be resolved by Western Blot.\u003cbr\u003e\nRRID: AB_2924617 (BioLegend Cat. No. 606451) AB_2924617 (BioLegend Cat. No. 606452)\u003cbr\u003e\nStructure: SMAD2 is a 467 amino acid protein with a predicted molecular weight of 52 kD. SMAD2 is very similar in sequence and structure to SMAD3.\u003cbr\u003e\nDistribution: Non-phosphorylated SMAD2 is present in the cellular cytoplasm close to the TGF-B receptor. Phosphorylated SMAD2 is present in the cellular nucleus bind to target promotor sequences.\u003cbr\u003e\nFunction: The function of SMAD2 is mainly as a gene expression regulator. SMAD2 acts as a sequence specific transcriptional repressor.\u003cbr\u003e\nInteraction: Non-phosphorylated SMAD2 interacts with SARA (SMAD anchor for receptor activation) protein. Phosphorylated SMAD2 interacts with SMAD4 and other members of the transcription repressor complex.\u003cbr\u003e\nLigand\/Receptor: SMAD2 is part of the TGF-B signaling pathway but it is not a ligand\/receptor protein\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cell Cycle\/DNA Replication, Signal Transduction, Transcription Factors\u003cbr\u003e\nMolecular Family: Phospho-Proteins\u003cbr\u003e\nGene ID: 4087\u003cbr\u003e\nUniProt: View information about SMAD2 on UniProt.org\u003cbr\u003e\nClone: 6H5-E3-C11\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: SMAD family member 2, CHTD8, hMAD-2, JV18-1, LDS6, MADH2, MADR2, HSMAD2MAD Homolog 2\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862480146601,"sku":"606451","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-606451","provider":"Iright","version":"1.0","type":"link"}