{"product_id":"biolegend-606759","title":"Biolegend, 606759, Alexa Fluor® 647 anti-Avi-tag Antibody, 25tests","description":"\u003cp\u003eAvi-tag is a 15 amino acid sequence, and the minimal acceptor peptide of a larger protein domain that serves as a substrate for the biotin ligase BirA from E. coli . Although one of the major advantages of Avi-tag fusion proteins is a controlled biotinylation in this domain, Avi-tag is also a useful tool in cell and molecular biology.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Epitope tag\u003cbr\u003e\nReported Reactivity: Species independent\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nFormulation: µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.\u003cbr\u003e\nConcentration: µg sizes: 0.5 mg\/mLtest sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC (test sizes) - Quality tested ICC (µg size) - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of the test-sized antibody is quality control tested by intracellular flow cytometry using our Cyto-Fast™ Fix\/Perm Buffer Set. For flow cytometric staining, the suggested use of this reagent is 5 µL per 106 cells in 100 µL volume. It is highly recommended that the reagent be titrated for optimal performance for each application. Each lot of the µg-sized antibody is quality control tested by immunocytochemistry. For immunocytochemistry using the ug size, a concentration range of 1.25 - 5.0 μg\/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm \/ 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: This clone recognizes proteins tagged with Avi-tag sequence irrespective of its biotinylation status.\u003cbr\u003e\nAdditional Product Notes: ICFC: Cells were fixed and permeabilizes using Cyto -Fast™ Buffer system ICC: Cells were fixed with 4% PFA and permeabilized with Triton X-100\u003cbr\u003e\nRRID: AB_3068042 (BioLegend Cat. No. 606759) AB_3068042 (BioLegend Cat. No. 606762) AB_3068042 (BioLegend Cat. No. 606760)\u003cbr\u003e\nStructure: GLNDIFEAQKIEWHE. Avi-tag amino acid sequence\u003cbr\u003e\nDistribution: Depends on the subcellular location of the tagged protein\u003cbr\u003e\nBiology Area: Cell Biology, Protein Purification, Protein Trafficking and Clearance\u003cbr\u003e\nGene ID: NA\u003cbr\u003e\nUniProt: View information about Avi-tag on UniProt.org\u003cbr\u003e\nClone: W19258A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Avi-Tag, AviTag, Avi epitope Tag\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866413650089,"sku":"606759","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-606759","provider":"Iright","version":"1.0","type":"link"}