{"product_id":"biolegend-612253","title":"Biolegend, 612253, Biotin anti-human CCL1 Antibody, 50μg","description":"\u003cp\u003eCCL1 was initially identified as a secreted protein derived from activated T cells. The CCL1\/CCR8 axis is involved in the recruitment of Th2 effector cells in vivo to sites of allergic mucosal inflammation. As a result, there is an increase of CCR8+ CD4 T cell numbers in allergic asthma and a higher concentration of CCL1 in bronchoalveolar lavage of asthmatic patients compared with normal controls. In addition, high levels of CCL1 have been detected in the serum of patients with atopic dermatitis. CCL1 is constitutively expressed in normal skin, and CD8 and CD4 CCR8+ cells have been isolated from normal skin. Therefore, it has been suggested that CCL1\/CCR8 play a role in skin immunosurveillance. A more recent publication showed that CCL1 transcript is upregulated in CD4 converted to Tregs by TGF-β in vitro , and this transcriptional upregulation is reversed by IL-6. This data suggests that CCL1 plays a role in Treg conversion. Antibodies against CCL1 also inhibit the suppressive function of Tregs. Furthermore, the antitumor effect of CpG-ODN plus anti-CCL1 in BALB-neuT mice suggest that neutralization of CCL1 can be used as an adjuvant to antitumor immunotherapy.\u003cbr\u003e\n50μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Human Recombinant CCL1 Protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.\u003cbr\u003e\nApplication: Direct ELISA - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Direct ELISA. The optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user.\u003cbr\u003e\nAdditional Product Notes: This product is intended for sandwich-based immunoassay development on various assay platforms requiring antibody pairs. This antibody (clone A16001C) could function as a detection antibody when paired with purified anti-human CCL1 (I-309) antibody (Cat. No. 613252) (clone A16001E) as the capture antibody and using Recombinant Human CCL1 (I-309) (carrier-free) protein (Cat. Nos. 582702) (Cat. Nos. 582702, 582704, 582706, and 582708) as the assay standard.\u003cbr\u003e\nRRID: AB_2941607 (BioLegend Cat. No. 612253)\u003cbr\u003e\nStructure: Chemokine\u003cbr\u003e\nDistribution: T cells, Th1, Th2, diabetogenic T cells, peritoneal macrophages, peritoneal mesothelial cells, Langerhans cells, monocytes, mast cells, NK cells, endothelial cells, and epithelial cells\u003cbr\u003e\nFunction: CCL1 chemoattracts CD4 Th2 cells and plays a key role in the progression of Th2 type hypersensitivity.\u003cbr\u003e\nInteraction: T cells, Th2 cells, macrophages, microglia, FOXP3+ CD4 regulatory T cells, skinhoming CLA+T cells, monocytes, NK cells, dendritic cells, endothelial cells, and smooth muscle cells\u003cbr\u003e\nLigand\/Receptor: CCR8 (CDw198)\u003cbr\u003e\nBiology Area: Cell Biology, Signal Transduction\u003cbr\u003e\nMolecular Family: Cytokines\/Chemokines\u003cbr\u003e\nAntigen References: Miller MD, et al. 1989. J Immunol. 143:2907-16. Haque NS, et al. 2004. Blood. 103:1296-304. Gombert M, et al. 2005. J Immunol. 174:5082-91. Montes-Vizuet R, et al. 2006. Eur Respir J. 28:59-67. Soler D, et al. 2006. J Immunol. 177:6940-51. Gonzalo JA, et al. 2007. J Immunol. 179:1740-50. Mutalithas K, et al. 2010. Clin Exp Allergy. 40:1175-85.\u003cbr\u003e\nGene ID: 6346\u003cbr\u003e\nUniProt: View information about CCL1 on UniProt.org\u003cbr\u003e\nClone: A16001C\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: T lymphocyte-secreted protein I-309\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003cbr\u003e\nQ: How many biotin molecules are per antibody structure?\u003cbr\u003e\nA: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46864391307433,"sku":"612253","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-612253","provider":"Iright","version":"1.0","type":"link"}