{"product_id":"biolegend-614854","title":"Biolegend, 614854, Alexa Fluor® 488 anti-α Smooth Muscle Actin Antibody, 100μg","description":"\u003cp\u003eCytoplasmic actins, which belong to the microfilament system of cytoskeleton proteins, are some of the most conserved eukaryotic proteins expressed in mammals. α-smooth muscle actin is widely expressed in contractile cells, most notably the mural cells that line arteries and arterioles. Overexpression of α-smooth muscle actin is a hallmark of fibrosis, a thickening or scarring of damaged tissue.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: N-terminal synthetic peptide of alpha-smooth muscle actin coupled to keyhole limpet haemocyanin (KLH).\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: IHC-P - Quality testedICC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 µg\/mL is suggested. For immunocytochemistry, a concentration range of 5.0 - 10.0 μg\/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: For use in immunohistochemistry (IHC) on formalin-fixed paraffin-embedded tissues, we recommend heat-induced antigen retrieval with either Tris-EDTA pH 9.0 or Citrate Buffer, 10X (Cat. No. 420902).For use in immunocytochemistry (ICC), we recommend fixation with Fixation Buffer (Cat. No. 420801) followed by permeabilization with 0.5% Triton-X.Previously Covance catalog# MMS-466S.\u003cbr\u003e\nRRID: AB_3662308 (BioLegend Cat. No. 614853) AB_3662308 (BioLegend Cat. No. 614854)\u003cbr\u003e\nStructure: α smooth muscle actin is a 377 amino acid protein with a predicted molecular weight of 42 kD.\u003cbr\u003e\nDistribution: Cytoplasmic, cytoskeleton\u003cbr\u003e\nFunction: Cell motility\u003cbr\u003e\nInteraction: α smooth muscle actins bind to myosins to produce motive force\u003cbr\u003e\nCell Type: Mesenchymal Stem Cells, Monocytes\u003cbr\u003e\nBiology Area: Cell Biology, Cell Motility\/Cytoskeleton\/Structure, Cell Structure, Neuroscience, Neuroscience Cell Markers\u003cbr\u003e\nMolecular Family: Microfilaments\u003cbr\u003e\nAntigen References: Brennan PA, et al. 2000. J Oral Pathol Med. 29:279-83. Rizeq MN, et al. 1994. Hum Pathol. 25:671-7. Schmitt-Graff A, et al. 1991. Am J Pathol. 138::1233-42 Roholl PJM, et al. 1990. Hum Pathol. 21:1269-74. Alberts B, et al. 1989. Eds Molecular biology of the cell. 2:613. Skalli O, et al. 1986. J Cell Biol. 103:2787-96.\u003cbr\u003e\nGene ID: 59\u003cbr\u003e\nUniProt: View information about alpha Smooth Muscle Actin on UniProt.org\u003cbr\u003e\nClone: 1A4\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Actin, aortic smooth muscle (αSMA), alpha 2 actin (ACTA2), alpha-cardiac actin, growth-inhibiting gene 46, cell growth-inhibiting gene 46 protein\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860833685673,"sku":"614854","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-614854","provider":"Iright","version":"1.0","type":"link"}