{"product_id":"biolegend-619302","title":"Biolegend, 619302, Purified anti-TIF1β (KAP-1, TRIM28) Antibody, 100μg","description":"\u003cp\u003eTIFβ (transcription intermediary factor 1-beta) is an 88 kD member of the tripartite motif family. This protein contains three zinc binding domains, a RING domain, a B-box type 1 and type 2 domain, and a coiled-coil region. TIFβ is found in the nucleus and associates with specific chromatin regions. This protein forms a complex with KRAB-domain transcription factors and recruits SETDB1 to histone 3 to increase KRAB-mediated transcriptional repression. TIF1β has been reported to interact with SETDB1 and CBX3 proteins. Studies using knockout mice reveal the important function of TIF1β in regulating genomic imprinting, T cell activation, and T cell tolerance.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nFormulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg\/ml.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: Upon receipt, store between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested ICC - Verified ChIP - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.025 - 0.1 µg per ml. For immunocytochemistry, a concentration range of 2.5 - 10 μg\/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunofluorescence1, Western blotting1 and chromatin immunoprecipitation1. This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing). This clone may produce increased background by western blot when used at concentrations exceeding 0.1 µg\/mL.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Chang CW, et al. 2008 BMC Mol. Biol. 9:61. (WB, IF, ChIP)\u003cbr\u003e\nProduct Citations: Yang DM, et al. 2024. Nat Commun. 780:15. PubMed Ouyang C, et al. 2022. Int J Biol Sci. 18:2655. PubMed\u003cbr\u003e\nRRID: AB_2209724 (BioLegend Cat. No. 619301) AB_2209723 (BioLegend Cat. No. 619302)\u003cbr\u003e\nStructure: Tripartite motif family, contains three zinc-binding domains, a RING, a B-box type 1 and 2 domain, and a coiled-coil region. Predicted molecular weight approximately 110 kD\u003cbr\u003e\nDistribution: Nuclear, associates with specific chromatin regions\u003cbr\u003e\nFunction: Forms a complex with KRAB-domain transcription factor and recruits SETDB1 to histone 3 to increase KRAB-mediated transcriptional repression\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Transcription Factors\u003cbr\u003e\nMolecular Family: Protein Kinases\/Phosphatase\u003cbr\u003e\nAntigen References: 1. Ryan RF, et al. 1999. Mol. Cell. Biol. 19:4366. 2. Schultz DC, et al. 2002. Genes Dev. 16:919. 3. Moosmann PR, et al. 1996. Nucleic Acids Res. 24:4859. 4. Friedman JR, et al. 1996. Genes Dev. 10:2067. 5. Messerschmidt DM, et al. 2012. Science 335:1499. 6. Chikuma S, et al. 2012. Nat. Immunol. 13:596.\u003cbr\u003e\nGene ID: 10155\u003cbr\u003e\nUniProt: View information about TIF1beta on UniProt.org\u003cbr\u003e\nClone: 20A1\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Transcription intermediary factor 1-beta, KRAB-associated protein, TRIM28, KAP-1\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860868616361,"sku":"619302","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-619302","provider":"Iright","version":"1.0","type":"link"}