{"product_id":"biolegend-619602","title":"Biolegend, 619602, Purified anti-4EBP1 Phospho (Thr70) Antibody, 100μg","description":"\u003cp\u003eIn eukaryotes, the mTOR kinase signaling pathway links cell growth and proliferation with nutrient availability. Under nutrient-deplete conditions, eukaryotic translation initiations factor 4E-binding protein 1 (4EBP1) binds the general translation factor eIF4E, resulting in an attenuation of cap-dependent translation. Under nutrient-replete conditions, mTORC1 and mTORC2 complexes phosphorylate 4EBP1 at Threonine 70 and disrupt the formation of the 4EBP1-eIF4E complex, thereby alleviating the repressive function of 4EBP1.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Synthetic peptide corresponding to human 4EBP1 phosphorylated at Threonine 70\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: This clone has not been tested for ICC and IP.\u003cbr\u003e\nRRID: AB_2814468 (BioLegend Cat. No. 619601) AB_2814469 (BioLegend Cat. No. 619602)\u003cbr\u003e\nStructure: 4EBP1 is a 118 amino acid protein with a predicted molecular weight of 13 kD.\u003cbr\u003e\nDistribution: Cytosol and nucleus\/ubiquitously expressed\u003cbr\u003e\nFunction: Regulation of global translation\u003cbr\u003e\nBiology Area: Cell Biology, Cell Proliferation and Viability, Protein Synthesis, Signal Transduction\u003cbr\u003e\nMolecular Family: Phospho-Proteins\u003cbr\u003e\nAntigen References: Brunn GJ, et al. 1997. Nature. 277:99. Hara K, et al. 2002. Cell. 110:177. Ekim B, et al. 2011. Mol. Cell Biol. 31:2787.\u003cbr\u003e\nGene ID: 1978\u003cbr\u003e\nUniProt: View information about 4EBP1 on UniProt.org\u003cbr\u003e\nClone: A18010A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Eukaryotic Translation Initiation Factor 4E Binding Protein 1, Phosphorylated Heat- And Acid-Stable Protein Regulated By Insulin 1, PHAS-I\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866058805417,"sku":"619602","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-619602","provider":"Iright","version":"1.0","type":"link"}