{"product_id":"biolegend-620302","title":"Biolegend, 620302, Purified anti-SHP-1 Antibody, 100μg","description":"\u003cp\u003eSHP-1, also referred to as PTPN6 is an inhibitory protein phosphatase widely expressed in hematopoietic cell lineages that regulates cell cycle, proliferation, and inflammatory pathways. SHP-1 dephosphorylates activating signaling molecules in receptor tyrosine kinase pathways; in T cells it functions as a negative regulator of T cell activation and proliferation. SHP-1 is constitutively bound to the ITIM-containing adaptor molecules such as the inhibitory receptor LAIR-1; activation of SHP-1 results in either direct dephosphorylation of the T cell receptor or downstream effector molecules such as Zap70 and Lck. Reduced expression of SHP1 is found in leukemias, lymphomas, and colorectal cancers due to promoter hypermethylation of the PTPN6 gene.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Partial recombinant protein corresponding to amino acid residues 1-213 of human SHP-1\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested ICC, IP, ICFC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg\/mL is recommended. For immunoprecipitation, the suggested use of this reagent is 2.5 µg per mL. For intracellular flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: W17240D was tested for ICC using PFA-fixed Jurkat cells permeabilized with either Triton X-100 or methanol. While both permeabilization methods facilitated SHP-1 staining, methanol resulted in considerably stronger staining.\u003cbr\u003e\nProduct Citations: Tathe P, et al. 2022. EMBO J. 41:e109720. PubMed Lam W, et al. 2023. Front Pharmacol. 13:1095186. PubMed Huang CY, et al. 2022. Int J Mol Sci. 23:. PubMed\u003cbr\u003e\nRRID: AB_2810679 (BioLegend Cat. No. 620301) AB_2810680 (BioLegend Cat. No. 620302)\u003cbr\u003e\nStructure: SHP-1 is a 595 amino acid protein with a predicted molecular weight of 68 kD.\u003cbr\u003e\nDistribution: Cytoplasm and nucleus\/hematopoietic cells\u003cbr\u003e\nCell Type: T cells, Thymocytes\u003cbr\u003e\nBiology Area: Cell Biology, Cell Proliferation and Viability, Immuno-Oncology, Immunology\u003cbr\u003e\nMolecular Family: Protein Kinases\/Phosphatase\u003cbr\u003e\nAntigen References: Paulson RF, et al. 1996. Nat Genet. 13:309. Khoury JD, et al. 2004. Blood. 104:1580. Paster W, et al. 2015. EMBO J. 34:393.\u003cbr\u003e\nGene ID: 5777\u003cbr\u003e\nUniProt: View information about SHP-1 on UniProt.org\u003cbr\u003e\nClone: W17240D\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Protein Tyrosine Phosphatase, Non-Receptor Type 6, PTPN6, Hematopoietic Cell Protein-Tyrosine Phosphatase, Protein-Tyrosine Phosphatase SHP-1, Protein-Tyrosine Phosphatase 1C\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866327896233,"sku":"620302","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-620302","provider":"Iright","version":"1.0","type":"link"}