{"product_id":"biolegend-621602","title":"Biolegend, 621602, Purified anti-human CD279 (PD-1) Antibody, 100μg","description":"\u003cp\u003eProgrammed cell death protein 1 (PD-1), also known as CD279, is a 55 kD member of the immunoglobulin superfamily. CD279 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic region and plays a key role in peripheral tolerance and autoimmune disease. CD279 is expressed predominantly on activated T cells, B cells, and myeloid cells. PD-L1 (B7-H1, CD274) and PD-L2 (B7-DC, CD273) are ligands of CD279 (PD-1) and are members of the B7 gene family. Evidence suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. Interaction of CD279 ligands results in inhibition of T cell proliferation and cytokine secretion.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Recombinant human CD279 protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested Direct ELISA - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. For Direct ELISA, the optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: A17188B antibody can block the binding of NAT105 and EH12.2H7 antibodies to the target.\u003cbr\u003e\nProduct Citations: Le X, et al. 2021. J Thorac Oncol. 16:583. PubMed\u003cbr\u003e\nRRID: AB_2820103 (BioLegend Cat. No. 621602)\u003cbr\u003e\nStructure: 55 kD type I transmembrane protein\u003cbr\u003e\nDistribution: Transiently expressed on CD4- and CD8- thymocytes, upregulated in thymocytes and splenic T and B lymphocytes, and is expressed on activated myeloid cells.\u003cbr\u003e\nFunction: Signaling and co-stimulation (co-inhibition)\u003cbr\u003e\nInteraction: SHP-1 and SHP-2\u003cbr\u003e\nLigand\/Receptor: PD-L1 (CD274) and PD-L2 (CD273)\u003cbr\u003e\nCell Type: Lymphocytes, T cells\u003cbr\u003e\nBiology Area: Cell Biology, Immuno-Oncology, Inhibitory Molecules\u003cbr\u003e\nMolecular Family: CD Molecules\u003cbr\u003e\nAntigen References: Ishida Y, et al. 1992. EMBO J. 11:3887 Francisco LM, et al. 2010. Immunol Rev. 236:219\u003cbr\u003e\nGene ID: 5133\u003cbr\u003e\nUniProt: View information about CD279 on UniProt.org\u003cbr\u003e\nClone: A17188B\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: PD1, PDCD1\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866055528617,"sku":"621602","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-621602","provider":"Iright","version":"1.0","type":"link"}