{"product_id":"biolegend-623402","title":"Biolegend, 623402, Purified anti-Acetylated Lysine Antibody, 100μg","description":"\u003cp\u003eProteins are reversibly and dynamically acetylated on the ε-amino group of lysine by acetyltransferases and deacetylated by deacetylases. This post-translational modification can regulate protein function (interactions with other proteins and DNA binding). Histones and transcription factors (PCAF, p53, p300, etc) appear to be the major targets of acetyltransferases. Acetylation is usually associated with chromatin remodeling and transcriptional activation, although in some cases (telomeres) it is associated with gene silencing. The 15G10 antibody recognizes acetylated lysine residues on proteins.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Species independent\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Acetylated protein mixture\u003cbr\u003e\nFormulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: Upon receipt, store between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested ICC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg antibody per 5 ml antibody dilution buffer for each mini-gel. For immunocytochemistry: Use a dilution range of 1-4 µg\/ml. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Kuo HP, et al. 2010. Sci Signal. 3:Ra9. PubMed\u003cbr\u003e\nProduct Citations: Yang JM, et al. 2022. Sci Adv. 8:eabo0183. PubMed Zhang W, et al. 2022. J Clin Invest. 132: . PubMed Sun X, et al. 2022. J Am Heart Assoc. 11:e025266. PubMed Lee J, Homma T, Fujii J, et al. 2017. Toxicol Lett. 10.1016\/j.toxlet.2017.06.005. PubMed Wu Q, et al. 2021. J Immunother Cancer. 9: . PubMed Kuo H, et al. 2010. Sci Signal. 3:ra9. PubMed Chen G, et al. 2019. EMBO Mol Med. 11:e10469. PubMed Han S, et al. 2017. Nat Commun. 8:14568. PubMed\u003cbr\u003e\nRRID: AB_315967 (BioLegend Cat. No. 623401) AB_315968 (BioLegend Cat. No. 623402)\u003cbr\u003e\nStructure: Proteins are acetylated by the reversible transfer of acetyl-CoA to the ε-amino group of lysine\u003cbr\u003e\nFunction: Post-translational modification of proteins by acetyltransferases and deacetylated by deacetylases to regulate protein-protein and protein-DNA interactions\u003cbr\u003e\nBiology Area: Cell Biology, Chromatin Remodeling\/Epigenetics\u003cbr\u003e\nAntigen References: 1. Wang S, et al. 2004. Novartis Found. Symp. 259:238. 2. Legube G, et al. 2003. EMBO Rep. 4:944. 3. Chen LF, et al. 2003. J. Mol. Med. 81:549.\u003cbr\u003e\nGene ID: NA\u003cbr\u003e\nClone: 15G10\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Proteins are acetylated by the reversible transfer of acetyl-CoA to the ε-amino group of lysine\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860814155945,"sku":"623402","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-623402","provider":"Iright","version":"1.0","type":"link"}