{"product_id":"biolegend-624351","title":"Biolegend, 624351, Purified anti-MAVS Antibody, 25μg","description":"\u003cp\u003eMitochondrial antiviral signaling protein (MAVS) is essential and specific for innate immunity by activating IRF3, IRF7 and NF-κB in response to viral infection. MAVS is located on the outer membranes of mitochondria, peroxisomes, and mitochondria-associated endoplasmic reticulum membranes (MAM). The MAVS protein contains an N-terminal CARD domain and a C-terminal mitochondrial transmembrane domain. MAVS acts downstream of the RIG-I RNA helicase and sensing viral RNA, leading to the recruitment of IKKε, TRIF and TRAF6. Some viruses have developed strategies to evade these innate defense mechanisms by using proteases that cleave MAVS and prevent its localization to mitochondria. The C-terminal region of MAVS cleavage by the hepatitis C virus (HCV) protease allows HCV to evade the host immune system.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Partial Recombinant human MAVS protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested ICC, IHC-P - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg\/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg\/mL is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 0.5 - 5.0 µg\/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: The clone does not cross-react with mouse in western blotting (WB). For immunocytochemistry (ICC), we recommend to use 4% PFA fixation followed by permeabilization with 0.5% Triton X-100 or ice cold-methanol, or ice-cold methanol fixation. For immunohistochemistry (IHC-P), antigen retrieval with Citrate Buffer (Cat. No. 420901) or Tris-EDTA pH 9.0 is recommended.\u003cbr\u003e\nRRID: AB_3097498 (BioLegend Cat. No. 624351) AB_3097498 (BioLegend Cat. No. 624352)\u003cbr\u003e\nStructure: MAVS protein is a 540 amino acid protein with a predicted molecular weight of 57 kD\u003cbr\u003e\nDistribution: Ubiquitously expressed, with highest levels in heart, skeletal muscle, liver, placenta and peripheral blood leukocytes\u003cbr\u003e\nFunction: Innate immunity\u003cbr\u003e\nInteraction: RIGI, IFIH1\/MDA5, TRAF2, TRAF6, C1QBP, FADD, RIPK1, CHUK, IKBKB and NLRX1\u003cbr\u003e\nCell Type: Epithelial cells, Leukocytes, Monocytes, T cells\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: Innate Immune Signaling\u003cbr\u003e\nAntigen References: Seth RB, et al. 2005. Cell. 122:669-82. Sun Q, et al. 2006. Immunity. 24:633-42. Xu LG, et al. 2005. Mol Cell. 19: 727-40. Yoneyama M, et al. 2004. Nat Immunol. 5:730-7. Lin R, et al. 2006. J Virol. 80:6072-83. Chen Z, et al. 2007. J Virol. 81:964-76. Li XD, et al. 2005. PNAS. 102:17717-22.\u003cbr\u003e\nGene ID: 57506\u003cbr\u003e\nUniProt: View information about MAVS on UniProt.org\u003cbr\u003e\nClone: W21144C\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Interferon beta promoter stimulator protein 1, IPS-1, Virus-induced-signaling adapter, VISA, Mitochondrial Antiviral-Signaling Protein\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866430787753,"sku":"624351","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-624351","provider":"Iright","version":"1.0","type":"link"}