{"product_id":"biolegend-624502","title":"Biolegend, 624502, Purified anti-STAT2 Antibody, 200microl","description":"\u003cp\u003eSTAT2, a 113 kD member of the STAT (signal transducer and activators of transcription) protein family, is phosphorylated in response to interferon alpha receptor signaling by Janus kinase I. Phosphorylation of STAT 2 induces nuclear translocation to activate transcription. STAT2 is highly expressed in the skin and thymus. STAT 2 forms complex with STAT1 and p48 to activate transcription and does not bind DNA directly. STAT 3 interacts with interferon regulatory factor 9, interferon alpha receptor, STAT 1, p48, and CREB binding protein. The Poly6245 antibody recognizes the C-terminal region of human STAT2 and has been shown to be useful for Western blotting, immunoprecipitation, and immunohistochemistry.\u003cbr\u003e\n200microl\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Polyclonal\u003cbr\u003e\nHost Species: Rabbit\u003cbr\u003e\nImmunogen: Peptide mapping to the carboxy terminus of human STAT2\u003cbr\u003e\nFormulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% gelatin.\u003cbr\u003e\nPreparation: The antibody was purified by antigen-affinity chromatography.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. DO NOT FREEZE.\u003cbr\u003e\nApplication: WB - Quality tested IP - Reported in the literature, not verified in house\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. For IHC, use a 1:50 dilution of antibody for staining. Antigen retrieval for IHC of formalin-fixed paraffin-embedded tissue using 0.01 M sodium citrate buffer is recommended. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation. This product may contain other non-IgG subtypes.\u003cbr\u003e\nAdditional Product Notes: The 200 µL size can be used for approximately 20 Western blots.\u003cbr\u003e\nRRID: AB_2198452 (BioLegend Cat. No. 624502)\u003cbr\u003e\nStructure: STAT family, contains coiled coil and SH2 domain, 113 kD\u003cbr\u003e\nDistribution: Translocates into nucleus after phosphorylation. Expressed in skin, thymus.\u003cbr\u003e\nFunction: Phosphorylated in response to cytokine signaling by receptor-associated kinases, translocates to nucleus to act as transcriptional activator. Interferon alpha binding to its receptor induces a transcription complex (ISGF3) composed of p48, STAT2, and STAT\u003cbr\u003e\nInteraction: STAT1, p48, interferon regulatory factor 9, interferon receptor 2, CREB binding protein\u003cbr\u003e\nModification: Phosphorylation (Tyr 690) by Janus kinase 1\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Signal Transduction, Transcription Factors\u003cbr\u003e\nMolecular Family: Nuclear Markers\u003cbr\u003e\nAntigen References: 1. Bluyssen HAR, et al. 1997. J. Biol. Chem. 272:4600. 2. Qureshi AA, et al. 1996. Mol. Cell. Biol. 16:288.\u003cbr\u003e\nRegulation: Phosphorylated by signaling from IFN alpha receptor\u003cbr\u003e\nGene ID: 6773\u003cbr\u003e\nUniProt: View information about STAT2 on UniProt.org\u003cbr\u003e\nClone: Poly6245\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Signal transducer and activator of transcription 2, interferon alpha induced transcriptional activator\u003cbr\u003e\nIsotype: Rabbit Polyclonal IgG\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866470928553,"sku":"624502","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-624502","provider":"Iright","version":"1.0","type":"link"}