{"product_id":"biolegend-625702","title":"Biolegend, 625702, Purified anti-Oct-1 (POU2F1) Antibody, 100μg","description":"\u003cp\u003eOct-1 is a member of the Pit-Oct-Unc (POU) domain family of transcription factors, which are characterized by a bipartite POU domain. The POU domain that contains two DNA-binding subdomains; an N-terminal POU-specific subdomain and a C-terminal homeobox subdomain, each of which are tethered by a variable linker sequence. Oct-1 can function as both a transcriptional activator and repressor, and controls the expression of housekeeping genes such as those encoding Histone H2B and snRNPs, as well as genes involved in development and differentiation. The highly-modular POU domain enables Oct-1 to drive diverse transcriptional programming that occurs in a tissue-specific manner. The POU domains confers the flexibility for Oct-1 to function as both a monomer, dimer, and heterodimer, and also facilitates the interaction between Oct-1 with a broad set of transcriptional cofactor proteins. Moreover, Oct-1 undergoes multiple post-translational modifications that confers DNA-binding specificity to certain promoters, including phosphorylation, ubiquitination, and sumoylation. Oct-1 is highly-expressed in many types of cancers, and functionally different isoforms produced from alternative promoter usage have been reported.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Partial recombinant protein corresponding to human OCT-1 amino acid residues 251-500\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested IP - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. For immunoprecipitation, the suggested use of this reagent is 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: This clone was not tested for ICC Non-specific band present in Ramos at 60 kD\u003cbr\u003e\nRRID: AB_2814492 (BioLegend Cat. No. 625701) AB_2814493 (BioLegend Cat. No. 625702)\u003cbr\u003e\nStructure: Oct-1 is a 743 amino acid protein with a predicted molecular weight of 76kD\u003cbr\u003e\nDistribution: Nucleoplasm\/Ubiquitously expressed\u003cbr\u003e\nFunction: Transcription factor\u003cbr\u003e\nBiology Area: Cell Biology, Transcription Factors\u003cbr\u003e\nAntigen References: Pankratova EV, et al. 2018. Oncotarget. 9:29892 Strum RA and Herr W. et al. 1988. Nature. 336:601. Strum RA, et al. 1988. Genes Dev. 2:1582.\u003cbr\u003e\nGene ID: 5451\u003cbr\u003e\nUniProt: View information about Oct-1 on UniProt.org\u003cbr\u003e\nClone: W18054D\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: POU domain, class 2, transcription factor 1, Octamer-binding transcription factor 1, NF-A1\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866330058921,"sku":"625702","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-625702","provider":"Iright","version":"1.0","type":"link"}