{"product_id":"biolegend-626501","title":"Biolegend, 626501, Purified anti-Fyn Antibody, 25μg","description":"\u003cp\u003eFyn is a 59 kD protein tyrosine kinase and a member of the src subfamily. There are two reported isoforms of Fyn; FynT found in T cells, B cells and thymocytes, and FynB found in neuronal cells. Fyn has been shown to control cell growth, interact with the p85 subunit of PI3K, control high affinity IgE receptor-mediated mast cell degranulation, and participate in neuronal cell signaling. Fyn can be activated by T cell receptor engagement or anti-CD3 ligation. Fyn is known to be modified by phosphorylation and palmitoylation and has been shown to interact with Fyn binding protein, p85 PI3K, high affinity IgE receptor, and NCAM140. The FYN-59 monoclonal antibody recognizes human and mouse Fyn and has been shown to be useful for Western blotting, immunoprecipitation, and immunohistochemistry.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Partial recombinant human Fyn (aa 7-176) expressed in E coli\u003cbr\u003e\nFormulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg\/ml.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: Upon receipt, store antibody solution undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.125 - 0.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Laird RM, et al. 2010. J. Immunol. 185:6518. PubMed\u003cbr\u003e\nProduct Citations: Doi H, et al. 2014. Clin Immunol. 184:150. PubMed Nelson MH, et al. 2015. J Immunol. 1737:194. PubMed Wu L, et al. 2023. Cell Rep Med. 4:100917. PubMed Shi W, et al. 2020. J Biol Chem. 295:7653. PubMed Dwyer A, et al. 2016. J Leukoc Biol. 100: 163 - 175. PubMed Laird R, et al. 2010. J Immunol. 185:6518. PubMed Laird R, Hayes S 2010. PLoS One. 5:e8899. PubMed Lautz JD, et al. 2021. Cell Rep. 37:110076. PubMed Bland T, et al. 2019. Endocrinology. 161(2):. PubMed\u003cbr\u003e\nRRID: AB_2108785 (BioLegend Cat. No. 626501) AB_2278824 (BioLegend Cat. No. 626502)\u003cbr\u003e\nStructure: Protein tyrosine kinase family, src subfamily. Isoforms fynT, fynB, 59 kD\u003cbr\u003e\nDistribution: FynT T cells, thymocytes, B cells; fynB neuronal cells\u003cbr\u003e\nFunction: Controls cell growth, interacts with p85 subunit of PI3K. Involved in high affinity IgE receptor-mediated mast cell degranulation\u003cbr\u003e\nInteraction: Fyn binding protein, p85 PI3K, high affinity IgE receptor, NCAM140\u003cbr\u003e\nModification: Phosphorylation, Palmitoylation\u003cbr\u003e\nCell Type: T cells, Thymocytes, B cells\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Signal Transduction\u003cbr\u003e\nMolecular Family: Protein Kinases\/Phosphatase\u003cbr\u003e\nAntigen References: 1. Goldsmith J, et al. 2002. Biochem. Biophys. Res. Comm. 298:501. 2. Prasad K, et al. 1993. P. Natl. Acad. Sci. USA 90:7366. 3. Kawakami T, et al. 1986. Mol. Cell. Biol. 6:4195.\u003cbr\u003e\nRegulation: Activated by TCR engagement or anti-CD3\u003cbr\u003e\nGene ID: 2534\u003cbr\u003e\nUniProt: View information about Fyn on UniProt.org\u003cbr\u003e\nClone: FYN-59\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Tyrosine kinase p59fyn, Protein-tyrosine kinase fyn, Proto-oncogene tyrosine-protein kinase fyn\u003cbr\u003e\nIsotype: Mouse IgG\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866453069993,"sku":"626501","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-626501","provider":"Iright","version":"1.0","type":"link"}