{"product_id":"biolegend-627908","title":"Biolegend, 627908, Alexa Fluor® 647 anti-Tubulin-α Antibody, 100μg","description":"\u003cp\u003eTubulin, one of the major constituents of microtubules, is a dimeric protein consisting of an alpha and beta chain. Tubulin is a highly conserved protein that is ubiquitously expressed. Tubulin is a GTP-binding protein that can be modified by phosphorylation and acetylation. Tubulin interacts with a variety of proteins including RAC GTPase activating protein 1, ZAP70, Polo-like kinase, c-Myc, Fyn, and microtubule associated protein 1A. The 10D8 antibody recognizes α-tubulin in all species and is useful for Western blotting, and immunofluorescence staining. The 10D8 antibody can also be used to monitor protein loading in Western blotting.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse, Rat, All Species\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Recombinant full length human α-tubulin\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg\/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm \/ 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Chou HYE, et al. 2006. J. Biol. Chem. 281:15201.\u003cbr\u003e\nProduct Citations: Chatterjee S, et al. 2023. Sci Adv. 9:eade4800. PubMed Mei Y, et al. 2020. Nat Commun. 2.661111111. PubMed\u003cbr\u003e\nRRID: AB_2563178 (BioLegend Cat. No. 627908)\u003cbr\u003e\nStructure: Tubulin family, tubulin is a dimeric protein consisting of an alpha and beta chain. Alpha tubulin has an approximate molecular weight of 50-55 kD.\u003cbr\u003e\nDistribution: Ubiquitous\u003cbr\u003e\nFunction: Tubulin is one of the major constituents of microtubules. Binds GTP, alpha chain contains a non-exchangeable GTP binding site\u003cbr\u003e\nInteraction: RAC GTPase activating protein 1, ZAP70, Polo-like kinase, c-Myc, Fyn, Microtubule associated protein 1A\u003cbr\u003e\nModification: Phosphorylation, acetylation\u003cbr\u003e\nBiology Area: Cell Biology, Cell Motility\/Cytoskeleton\/Structure, Neuroscience, Neuroscience Cell Markers\u003cbr\u003e\nMolecular Family: Microtubules\u003cbr\u003e\nAntigen References: 1. Cowan NJ, et al. 1983. Mol. Cell. Biol. 3:1738. 2. Miller FD, et al. 1987. J. Cell Biol. 105:3065. 3. Wilde CD, et al. 1982. Proc. Natl. Acad. Sci. USA 79:96.\u003cbr\u003e\nGene ID: 84790\u003cbr\u003e\nUniProt: View information about Tubulin-alpha on UniProt.org\u003cbr\u003e\nClone: 10D8\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: α-tubulin, Tubulin, alpha-ubiquitous chain\u003cbr\u003e\nIsotype: Mouse IgM, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46860780077225,"sku":"627908","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-627908","provider":"Iright","version":"1.0","type":"link"}