{"product_id":"biolegend-628304","title":"Biolegend, 628304, Alexa Fluor® 647 anti-Lck Antibody, 100μg","description":"\u003cp\u003eLck is a 56 kD tyrosine protein kinase and a member of the src subfamily that contains both an SH2 and SH3 domain. Alternatively spliced isoforms of Lck have been reported. Lck is a cytoplasmic protein bound to CD4 and CD8 in T lymphocytes that participates in antigen-induced T cell activation through phosphorylation of the T cell receptor zeta chain. Lck is activated upon T cell receptor engagement and is involved in the onset of cell cycle progression induced by interleukin-2. Phosphorylation by Csk downregulates the activity of Lck. In addition to CD4 and CD8, Lck has been reported to interact with PI3K through its SH3 domain and tyrosine phosphorylated KHDRBS1\/p70 through its SH2 domain. In addition, Lck has been shown to associate with the SAP transmembrane tyrosine phosphatase. The Lck-01 monoclonal antibody recognizes human Lck and has been shown to be useful for Western blotting, immunoprecipitation, immunocytochemistry, and flow cytometry.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Peptide corresponding to a.a. 22-36 in human Lck\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality testedFC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining using our nuclear factor staining protocol. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm \/ 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: Does not cross-react with mouse.Clone may cross-react with other Src tyrosine kinase family members.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Romagnoli P, et al. 2001. Int. Immunol. 13:305.\u003cbr\u003e\nProduct Citations: Lui VG, et al. 2024. J Exp Med. :221. PubMed Bezverbnaya K, et al. 2021. Cytotherapy. 23:820. PubMed Shi Y, et al. 2020. Haematologica. . PubMed Reed JC, et al. 2020. PLoS One. 15:e0236921. PubMed\u003cbr\u003e\nRRID: AB_2564196 (BioLegend Cat. No. 628303) AB_2564197 (BioLegend Cat. No. 628304)\u003cbr\u003e\nStructure: Belongs to the tyrosine family of kinases, src subfamily. Contains one SH2 domain, 1 SH3 domain. Approximately 56 kD, alternatively spliced isoforms have been reported\u003cbr\u003e\nDistribution: Cytoplasmic protein, bound to cytoplasmic CD4 and CD8 in T lymphocytes\u003cbr\u003e\nFunction: Participates in antigen-induced T cell activation, phosphorylates zeta chain of T cell receptor. Involved in interleukin-2 induced onset of cell cycle progression\u003cbr\u003e\nInteraction: Associates with CD4 and CD8 co-receptors. Binds to PI3K through SH3 interactions, binds to tyrosine phosphorylated KHDRBS1\/p70 through SH2 domain. Interacts with SAP transmembrane tyrosine phosphatase\u003cbr\u003e\nModification: Phosphorylation\u003cbr\u003e\nCell Type: T cells\u003cbr\u003e\nBiology Area: Cell Biology, Signal Transduction\u003cbr\u003e\nMolecular Family: Protein Kinases\/Phosphatase\u003cbr\u003e\nAntigen References: 1. Koga Y, et al. 1986. Eur. J. Immunol. 16:1643. 2. Vogel LB, et al. 1995. Biochem. Biophys. Acta 1264:168. 3. Vogel LB, et al. 1993. Mol. Cell. Biol. 13:7408. 4. Bergaman M, et al. 1992. EMBO J. 11:2919.\u003cbr\u003e\nRegulation: Activated upon T cell receptor engagement. Tyrosine phosphorylation by p50 Csk downregulates catalytic activity\u003cbr\u003e\nGene ID: 3932\u003cbr\u003e\nUniProt: View information about Lck on UniProt.org\u003cbr\u003e\nClone: LCK-01\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Proto-oncogene tyrosine kinase lck, p56-Lck, T-cell specific protein tyrosine kinase\u003cbr\u003e\nIsotype: Mouse IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866459295913,"sku":"628304","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-628304","provider":"Iright","version":"1.0","type":"link"}