{"product_id":"biolegend-630051","title":"Biolegend, 630051, Purified anti-ATF-2 Antibody, 25μg","description":"\u003cp\u003eThe Activating Transcription Factor 2 (ATF-2), also known as CREB2 and CREBP1, contains a Basic Leucine Zipper (bZIP) domain and a C2H2-type zinc finger domain. ATF-2 binds to both DNA response factors AP-1 and CRE and belongs to the ATF\/CREB family of leucine zipper proteins. ATF-2 interacts with a variety of viral oncoproteins and cytosolic tumor suppressors and is a target of the SAPK\/JNK and p38 MAP kinase signaling pathways. ATF-2 activity can be initiated by various forms of cellular stress. Several studies have shown aberrant ATF-2 activation in the development and progression of mammalian skin tumors. ATF-2 may mediate carcinogenesis induced by the mutant Ras protein and regulate the maintenance of the invasive cancer phenotype of several epithelial cell types. ATF-2 has also been shown to be phosphorylated at its C-terminus (serine 472 and 480 in mice, serine 490 and 498 in human) by ATM during double-strand breaks. Mice with mutations of these two serines are sensitive to irradiation and more prone to tumorigenesis under p53 knockout condition.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Partial Recombinant human ATF2 protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested ICC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg\/mL. For immunocytochemistry, a concentration range of 2.0 - 10.0 μg\/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: The clone cross-reacts with mouse in western blot (WB). For immunocytochemistry (ICC), 4% PFA fixation followed by permeabilization with 0.5% Triton X-100 is recommended. This product is not recommended for use in immunohistochemistry (IHC).\u003cbr\u003e\nAdditional Product Notes: This antibody has been tested in knockout\/knockdown models for Western Blotting and Immunocytochemistry.\u003cbr\u003e\nRRID: AB_3097336 (BioLegend Cat. No. 630051) AB_3097336 (BioLegend Cat. No. 630052)\u003cbr\u003e\nStructure: ATF-2 protein is a 505 amino acid protein with a predicted molecular weight of 55 kD\u003cbr\u003e\nDistribution: Abundant expression in the brain\u003cbr\u003e\nFunction: Transcriptional activator\u003cbr\u003e\nInteraction: SMAD3, SMAD4, UTF1, NBN, MRE11, XPO1, KAT5, CUL3\u003cbr\u003e\nBiology Area: Cell Biology, DNA Repair\/Replication, Transcription Factors\u003cbr\u003e\nAntigen References: Abdel-Hafiz, H.A. et al. 1992. Mol Endocrinol. 6:2079-89. Gupta S, et al. 1995. Science. 267:389-93. Van Dam H, et al. 1995. EMBO J. 14:1798-811. Livingstone C, et al. 1995. EMBO J. 14:1785-97. Yamasaki T, et al. 2009. J Cell Biochem. 284:8567-81. Leslie MC, et al. 2009. J Cell Biochem. 94:25–38. Papassava P, et al. 2004. Cancer Res. 64:8573–84. Vlahopoulos SA, et al. 2008. BioEssays. 30:314–27. Bhoumik A, et al. 2005. Mol.Cell. 18:577–87. Li S, et al. 2010. Genes Cancer. 1:316–330.\u003cbr\u003e\nGene ID: 1386\u003cbr\u003e\nUniProt: View information about ATF-2 on UniProt.org\u003cbr\u003e\nClone: W21106A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: activating transcription factor 2 formerly, ATF2, cAMP-responsive element-binding protein 2, CREB2, CREBP1, CRE-BP1, cyclic AMP-dependent transcription factor ATF-2, HB16, MGC111558, TREB7\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866430034089,"sku":"630051","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-630051","provider":"Iright","version":"1.0","type":"link"}