{"product_id":"biolegend-631260","title":"Biolegend, 631260, Alexa Fluor® 647 anti-c-Fos Phospho (Ser32) (for IHC and ICC), 100μg","description":"\u003cp\u003eThe proto-oncogene c-Fos is the human homolog of the retroviral v-Fos oncogene, and is a member of the Fos family of transcription factors. c-Fos forms heterodimers with proteins of the Jun family of transcription factors to form the Activator Protein-1 complex (AP-1). This complex is involved in critical cellular processes such as inflammation, cell proliferation, differentiation, survival, hypoxia, bone development and angiogenesis which suggests c-Fos is an important therapeutic target for cancer, vascular remodeling, acute inflammation and autoimmune diseases.  In addition, increases in c-Fos expression and activation are associated with neuronal activity induced by psychoactive drugs. Expression of c-Fos is rapid and transitory, and phosphorylation at residues Ser32 and Thr232 by Erk5 increases protein stability and nuclear localization.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Synthetic peptide from human c-Fos phosphorylated at Ser32.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: IHC-P - Quality testedICC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg\/mL is suggested. For immunocytochemistry, a concentration range of 5.0 - 10.0 μg\/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm \/ 635 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses\u003cbr\u003e\nExcitation Laser: Red Laser (633 nm)\u003cbr\u003e\nApplication Notes: For ICFC use we recommend cell fixation\/permeabilization with True-Phos™ Perm buffer.\u003cbr\u003e\nRRID: AB_3662404 (BioLegend Cat. No. 631259) AB_3662404 (BioLegend Cat. No. 631260)\u003cbr\u003e\nStructure: c-Fos is a 380 amino acid length protein. It has a leucine zipper domain that allows it to form heterodimers with members of the Jun family proteins.\u003cbr\u003e\nDistribution: Non-phosphorylated forms can be found in the endoplasmic reticulum. However, after activation\/phosphorylation the protein is translocated to the nucleus.\u003cbr\u003e\nFunction: c-Fos is a subunit of the AP-1 transcription factor. The half-life of the protein is short; however, phosphorylation induces protein stability and translocation to the nucleus.\u003cbr\u003e\nInteraction: c-Fos interacts with members of the Jun family proteins to form the AP-1 transcription factor.\u003cbr\u003e\nLigand\/Receptor: As a part of the transcription factor AP-1, it binds to specific DNA sequences to regulate gene transcription\u003cbr\u003e\nBiology Area: Cell Biology, Cell Proliferation and Viability, Immuno-Oncology, Immunology, Innate Immunity, Neuroscience, Transcription Factors\u003cbr\u003e\nMolecular Family: Innate Immune Signaling, Phospho-Proteins, Tumor Suppressors\u003cbr\u003e\nAntigen References: Sasaki T, et al. 2006. Mol Cell. 24:63-75. Huang K, et al. 2014. Cell Signal. 26:629-38. Qu X, et al. 2019. Cancer Sci. 110:3183-3196. Guo JC, et al. 2015. PLoS One. 10:e0120332. Rodriguez-Berdini L, et al. 2020. J Biol Chem. 295:8808-8818.\u003cbr\u003e\nGene ID: 2353\u003cbr\u003e\nUniProt: View information about c-Fos on UniProt.org\u003cbr\u003e\nClone: A22009C\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: G0S7, p55, cFos, c-Fos, Proto-oncogene, Transcription Factor subunit, AP-1\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866076172457,"sku":"631260","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-631260","provider":"Iright","version":"1.0","type":"link"}