{"product_id":"biolegend-631352","title":"Biolegend, 631352, Purified anti-CD102 (ICAM-2) Antibody, 100μg","description":"\u003cp\u003eCD102 (ICAM-2) is a 55 kD type I transmembrane glycoprotein which belongs to the immunoglobulin superfamily. It is also known as Intercellular Adhesion Molecule-2, or ICAM-2. CD102 is constitutively expressed on vascular endothelial cells, some lymphocytes, monocytes, and platelets. CD102 is thought to be involved in lymphocyte recirculation, and in co-stimulation of the immune response. CD102 and CD54 in endothelial cells, such as in blood-brain barrier, are essential for assisting T cell polarization and crawling and diapedesis. CD102 is a counter receptor of LFA-1 (CD11a\/CD18), DC-SIGN and MAC-1.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Human CD102 (ICAM-2) recombinant protein fragment\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: FC - Quality tested ICC, WB - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. For immunocytochemistry, a concentration range of 1 - 10 μg\/mL is recommended. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg\/mL. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: For western blotting (WB) it is recommended to heat the cell lysate at 37 °C for 30 min rather than boiling it. For immunocytochemistry (ICC), either Fixation Buffer (Cat. No. 420801) or 4% paraformaldehyde followed by methanol permeabilization is recommended. The reactivity of this antibody (clone W22039C) in cell surface flow cytometry is able to be blocked by another BioLegend antibody (clone CBR-IC2\/2) (Cat. No. 328502).\u003cbr\u003e\nRRID: AB_3097547 (BioLegend Cat. No. 631351) AB_3097547 (BioLegend Cat. No. 631352)\u003cbr\u003e\nStructure: 55 kD type I transmembrane glycoprotein, Ig superfamily\u003cbr\u003e\nDistribution: Vascular endothelial cells, lymphocytes, monocytes, platelets\u003cbr\u003e\nFunction: Adhesion, costimulation\u003cbr\u003e\nLigand\/Receptor: LFA-1, DC-SIGN, MAC-1\u003cbr\u003e\nCell Type: Endothelial cells, Lymphocytes, Monocytes, Platelets\u003cbr\u003e\nBiology Area: Immunology\u003cbr\u003e\nMolecular Family: Adhesion Molecules, CD Molecules\u003cbr\u003e\nAntigen References: Weber KSC, et al. 2004. Inflammation. 28:177-88. Barclay AN, et al. 1997. The Leukoycte Antigen Factsbook. Casasnovas JM, et al. 1999. Proc Natl Acad Sci USA. 96:3017-22. Diamonds MS, et al. 1990. J Cell Biol. 111:3129-39. Marchetti L \u0026amp; Engelhardt B. 2020. Vasc Biol. 2:H1-H18.\u003cbr\u003e\nGene ID: 3384\u003cbr\u003e\nUniProt: View information about CD102 on UniProt.org\u003cbr\u003e\nClone: W22039C\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Intercellular Adhesion Molecule-2, ICAM-2\u003cbr\u003e\nIsotype: Mouse IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866432131241,"sku":"631352","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-631352","provider":"Iright","version":"1.0","type":"link"}