{"product_id":"biolegend-632051","title":"Biolegend, 632051, Purified anti-IRAK1 Antibody, 25μg","description":"\u003cp\u003eThe interleukin-1 receptor-associated kinase (IRAK) family of proteins are made up of serine\/threonine-specific kinases which play a critical role in the response to pathogens through the induction of acute inflammation and subsequent adaptive immune responses. The mammalian IRAK molecular family consists of four members (IRAK1, IRAK2, IRAK3\/IRAK-M, and IRAK4). Two are active kinases, IRAK1 and IRAK4, and two are inactive kinases, IRAK2 and IRAKM. However, all are involved in the regulation of the nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) pathways. The binding of IL-1 to IL-1 receptor type I (IL-1RI) initiates the formation of a complex that includes IL-1R1, IL-1RAcP, MyD88, and IRAKs. Currently, there are three differentially spliced variants of IRAK1: IRAK1, IRAK1b, and IRAK1c. Auto-phosphorylation plays a role in IRAK-1 activation and mediates proteasome-mediated degradation leading to IRAK1 protein loss. Meanwhile, IRAK1b lacks kinase activity and is resistant to proteasome-mediated degradation. Additionally, IRAK1c has a truncated sequence and is therefore mutated at the C-terminus of its kinase domain and acts as a negative regulator of the TLR and IL-1R signaling pathways.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Partial recombinant human IRAK1 protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested ICC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg\/mL. For immunocytochemistry, a concentration range of 1 - 5 μg\/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: For immunocytochemistry (ICC), we recommend using 4% PFA fixation followed by permeabilization with 0.5% Triton X-100 or ice cold-methanol, or ice-cold methanol fixation. This product is not recommended for use in immunohistochemistry (IHC).\u003cbr\u003e\nAdditional Product Notes: This antibody has been tested in knockout\/knockdown models for Western Blotting (WB).\u003cbr\u003e\nRRID: AB_3097576 (BioLegend Cat. No. 632051) AB_3097576 (BioLegend Cat. No. 632052)\u003cbr\u003e\nStructure: IRAK1 protein is a 712 amino acid protein with a predicted molecular weight of 77 kD\u003cbr\u003e\nDistribution: Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2.\u003cbr\u003e\nFunction: Serine\/threonine protein kinase\u003cbr\u003e\nInteraction: TRAF6, PELI1, IRAK4, MYD88, TOLLIP, IL1RL1, IKBKG\/NEMO, IRAK4, PELI3, INAVA and NFATC4\u003cbr\u003e\nCell Type: Macrophages, Monocytes\u003cbr\u003e\nBiology Area: Cell Biology, Immunology, Innate Immunity\u003cbr\u003e\nMolecular Family: Protein Kinases\/Phosphatase\u003cbr\u003e\nAntigen References: Dinarello CA. 1996. Blood. 87:2095-147. Takaesu G, et al. 2001. Mol Cell Biol. 21: 2475-84. Janssens S \u0026amp; Beyaert R. 2003. Mol Cell. 11:293-302. Jain A, et al. 2014. Front Immunol. 5:553. Gottipati S, et al. 2008. Cell Signal. 20:269-76.\u003cbr\u003e\nGene ID: 3654\u003cbr\u003e\nUniProt: View information about IRAK1 on UniProt.org\u003cbr\u003e\nClone: W22166E\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: AA48924; Il1rak; Interleukin 1 receptor associated kinase 1; IRAK; IRAK-1\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866434097321,"sku":"632051","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-632051","provider":"Iright","version":"1.0","type":"link"}