{"product_id":"biolegend-633001","title":"Biolegend, 633001, Purified anti-Cbl Antibody, 125microl","description":"\u003cp\u003eThe proto-oncogene c-Cbl was initially identified as the cellular homologue of v-Cbl oncogene that induces pre-B cell lymphomas and myeloid leukemias in mice. The Cbl family comprises three mammalian proteins, c-Cbl, Cbl-b, and Cbl-3; among these, c-Cbl and Cbl-b are expressed in hemopoietic cells, whereas Cbl-3 is expressed only in epithelial tissues. Cbl proteins can function as E3 ubiquitin (Ub) ligases, and in more recent studies Cbl has been implicated in the negative regulation of various RTKs.\u003cbr\u003e\n125microl\u003cbr\u003e\nVerified Reactivity: Human, Mouse, Rat\u003cbr\u003e\nAntibody Type: Polyclonal\u003cbr\u003e\nHost Species: Rabbit\u003cbr\u003e\nImmunogen: peptide mapping to the carboxy terminal domain of human Cbl\u003cbr\u003e\nFormulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% gelatin.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.\u003cbr\u003e\nApplication: WB - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting suggested working dilution(s): Use 5 µl per 5 ml antibody (1:1000) dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: This polyclonal was generated using an immunogen that corresponds to the C-terminus of c-Cbl. Due to poor sequence homology between the C-termini of c-Cbl and other Cbl family members, we do not predict this antibody will cross-react with other Cbl proteins. At concentrations greater than a 1:1000 dilution, this antibody may produce minor non-specific bands in certain lysates.\u003cbr\u003e\nAdditional Product Notes: The 125 µL size can be used for approximately 25 Western blots.\u003cbr\u003e\nRRID: AB_2070439 (BioLegend Cat. No. 633001)\u003cbr\u003e\nStructure: c-Cbl is a 906 amino acid protein with a predicted molecular weight of 99.6 kDa. The N-terminus is composed of the phosphotyrosine binding (PTB) domain, a short linker region and the RING-type zinc finger. C-terminal contains proline-rich domain with potential tyrosine phosphorylation sites.\u003cbr\u003e\nDistribution: Ubiquitous cytoplasmic protein\u003cbr\u003e\nInteraction: NCK, CD2AP, SLA, SLA2, EGFR, SYK, LAT2 and ZAP70\u003cbr\u003e\nBioactivity: E3 ubiquitin (Ub) ligases, and negative regulators of PTKs\u003cbr\u003e\nBiology Area: Cell Biology, Ubiquitin\/Protein Degradation\u003cbr\u003e\nAntigen References: 1. Liu YC, et al. 1998. Cell Signal 10:377.\u003cbr\u003e\nGene ID: 867\u003cbr\u003e\nUniProt: View information about Cbl on UniProt.org\u003cbr\u003e\nClone: Poly6330\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: E3 ubiquitin-protein ligase Cbl, Proto-oncogene c-Cbl\u003cbr\u003e\nIsotype: Rabbit Polyclonal IgG\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866471092393,"sku":"633001","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-633001","provider":"Iright","version":"1.0","type":"link"}