{"product_id":"biolegend-633508","title":"Biolegend, 633508, PE anti-Bcl-2 Antibody, 100μg","description":"\u003cp\u003eBcl-2 (B-cell leukemia 2) is an apoptotic protein and a member of the Bcl-2 family containing BH1-4 domains. Two reported isoforms exist α=25 kD; β=22 kD. The Bcl-2 protein forms homo- or hetero-dimers with other Bcl-2 family members. Bcl-2 is distributed in the outer mitochondrial membrane, the nuclear envelope, and the endoplasmic reticulum. This protein blocks apoptotic death by controlling mitochondrial membrane permeability. Cleavage of Bcl-2 can convert to pro-apoptotic (by cleavage of BH4 domain). Bcl-2 has been reported to regulate cell cycle progression via ROS. This protein is modified by ASK1\/JNK1, PKC, ERKs, and stress-activated kinase phosphorylation and can be ubiquitinated. Bcl-2 has been shown to interact with Apaf-1, Raf-1, TP53BP2, caspase-3, and form heterodimers with Bax, Bad, Bak, Bcl-x L , and Bag-1. Clone BCL\/10C4 has been shown to be useful for Western blotting, immunoprecipitation, and immunofluorescence of the mouse and rat Bcl-2 protein.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Mouse, Rat\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: N-terminal, Amino acid residues 61-76 of mouse Bcl-2\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.\u003cbr\u003e\nConcentration: 0.2 mg\/ml\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)Green Laser (532 nm)\/Yellow-Green Laser (561 nm)\u003cbr\u003e\nApplication Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue4, immunocytochemical staining5, and immunoprecipitation5.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Hsu YT, et al. 1997. J. Biol. Chem. 272:13829.Zuber J, et al. 2011. Genes Dev. 25:1628. (WB) PubMedDoi K, et al. 2012. Biochem Biophys Res Commun. 425:107. PubMedYamanaka, et al. 2003. J. Immunol. 170:816. (IHC)Conus S, et al. 2000. EMBO J. 19:1534. (WB, IP, ICC)\u003cbr\u003e\nProduct Citations: Cheng MI, et al. 2023. Nat Immunol. 24:780. PubMed Ozga AJ, et al. 2022. Immunity. 55:82. PubMed Stravokefalou V, et al. 2023. Front Immunol. 13:1014802. PubMed Oda SK, et al. 2020. J Exp Med. 217: . PubMed Sung BY, et al. 2022. J Clin Invest. 132:. PubMed Singh K, et al. 2015. Sci Rep. 14:7767. PubMed Ramos CV, et al. 2020. Cell Reports. 32(3):107910. PubMed Van Braeckel-Budimir N, et al. 2018. Cell Rep. 24:3374. PubMed Goh W, et al. 2020. Cell Rep. 33:108285. PubMed Engel I, et al. 2016. Nat Immunol. 10.1038\/ni.3437. PubMed Lehrke MJ, et al. 2021. Elife. 10:. PubMed Nakazawa Y, et al. 2021. Elife. 10:. PubMed Nabekura T, et al. 2020. Immunity. 96:52. PubMed Puleston D, et al. 2014. Elife. 3:3706. PubMed\u003cbr\u003e\nRRID: AB_2043939 (BioLegend Cat. No. 633507) AB_2290367 (BioLegend Cat. No. 633508)\u003cbr\u003e\nStructure: Apoptosis regulator proteins, Bcl-2 family, BH1-4 domains. Homo- or hetero-dimer; isoforms α, β 25 kD, 22 kD\u003cbr\u003e\nDistribution: Outer mitochondrial membrane, intracellular membrane nuclear envelope, endoplasmic reticulum\u003cbr\u003e\nFunction: Blocks apoptotic death by controlling the mitochondrial membrane permeability. Converted to pro-apoptotic activity by cleavage of BH4 domain. Regulates cell cycle progression via ROS\u003cbr\u003e\nModification: Phosphorylation, Ubiquitination\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cell Biology, Neuroscience\u003cbr\u003e\nAntigen References: 1. Tsujimoto Y, et al. 1986 P. Natl. Acad. Sci. USA 83:5214. 2. Yang E, et al. 1995. Cell 80:285. 3. Huang Z, et al. 2000. Oncogene 19:6627. 4. Deng X, et al. 2003. Blood. 102:3179.\u003cbr\u003e\nRegulation: Phosphorylation by ASK1\/JNK1, PKC, ERKs, stress-activated kinases\u003cbr\u003e\nGene ID: 596\u003cbr\u003e\nUniProt: View information about Bcl-2 on UniProt.org\u003cbr\u003e\nClone: BCL\/10C4\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: B-cell leukemia 2\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: What type of PE do you use in your conjugates?\u003cbr\u003e\nA: We use R-PE in our conjugates.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862905016489,"sku":"633508","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-633508","provider":"Iright","version":"1.0","type":"link"}